In vivo immunological suppression of spinal cord myelin development

Brain Research Bulletin
H S KeirsteadJ D Steeves

Abstract

The onset of myelination in the embryonic chick spinal cord begins on embryonic day (E) 12 or E13 of the 21 day in ovo developmental period. This event coincides with a loss of functional axonal regeneration following complete transection of the thoracic spinal cord. In this study, we have characterised an immunological method for delaying the developmental onset of myelination in vivo until later stages of development (developmental myelin-suppression). A single injection of heterologous or homologous serum complement proteins plus myelin-specific, complement-binding antibodies into the spinal cord prior to E13 delayed the onset of myelination until E17. The state of spinal cord myelin was assessed with immunohistochemical, histological and ultrastructural techniques. Northern blot analysis indicated that myelin basic protein mRNA was not down-regulated in myelin-suppressed spinal cords, which suggests that oligodendrocytes survived developmental myelin-suppression. Glial fibrillary acidic protein immunostaining of normal and treated tissue indicated that myelin-suppression did not alter the resident astrocyte population of the spinal cord or elicit astrogliosis. Immunostaining with microtubule-associated protein-2 and thionin...Continue Reading

References

Nov 9, 1979·Brain Research·S H DorfmanD H Silberberg
Jul 1, 1977·Journal of the Neurological Sciences·R A EamesW I McDonald
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H S KeirsteadJ D Steeves
Sep 1, 1990·Journal of Neurobiology·I ShimizuA Shneiderman
Jan 1, 1990·Annals of the New York Academy of Sciences·J A Benjamins, C A Dyer
Jan 1, 1985·Developmental Neuroscience·W B Macklin, C L Weill
Jan 1, 1989·Acta Neuropathologica·K OzawaM Kameyama
Nov 1, 1986·Neuropathology and Applied Neurobiology·D L Graça, W F Blakemore
Feb 1, 1987·Experimental Neurology·G N Sholomenko, J D Steeves
Oct 1, 1968·Journal of Neuropathology and Experimental Neurology·P W Lampert, S S Schochet
Jun 1, 1984·Journal of the Neurological Sciences·R C SergottR P Lisak
Jul 1, 1980·Transactions of the Ophthalmological Societies of the United Kingdom·R E Clifford-JonesW I McDonald
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RanschtW Seifert
May 1, 1961·The Journal of Biophysical and Biochemical Cytology·M B BUNGEH RIS
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Aug 23, 2008·Biomechanics and Modeling in Mechanobiology·David I ShreiberRagi A I Elias
Jan 23, 1999·Current Opinion in Neurobiology·B S Bregman
Feb 16, 2000·Progress in Retinal and Eye Research·M Dezawa, E Adachi-Usami
Aug 4, 2015·Frontiers in Molecular Neuroscience·Jason B CarmelRonald P Hart

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