PMID: 6988402Mar 1, 1980Paper

In vivo inactivation of glycerol dehydrogenase in Klebsiella aerogenes: properties of active and inactivated proteins

Journal of Bacteriology
F E RuchA L Goldberg

Abstract

Glycerol:oxidized nicotinamide adenine dinucleotide (NAD+) 2-oxidoreductase (EC 1.1.1.6), an inducible enzyme for anaerobic glycerol catabolism in Klebsiella aerogenes, was purified and found to have a molecular weight of 79,000 by gel electrophoresis. The protein seemed to be enzymatically active either as a dimer of a 40,000-dalton peptide at pH 8.6 or as a tetramer of 160,000 molecular weight at pH 7.0. The enzyme activity was present at high levels in cells growing anaerobically on glycerol, but disappeared with a half-life of about 45 min if molecular oxygen was introduced to the culture. In contrast, no such phenomenon occurred with dihydroxyacetone kinase activity, the second enzyme in the pathway. Immunochemical analysis showed that the inactivation of the oxidoreductase did not involve degradation of the protein. Furthermore, subunits of the active and inactive forms of the enzyme were indistinguishable in size on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and had similar isoelectric points (pH 4.7). Inactivation did, however, alter the gel filtration properties of the enzyme protein and, more importantly, reduced its affinity for the dye Cibacron F3GA and the coenzyme NAD+.

Citations

Jan 1, 1983·Journal of Molecular Evolution·R Z JinE C Lin
May 9, 1998·The Journal of Biological Chemistry·Z LuE C Lin
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pedro EchaveE C C Lin
Apr 12, 2011·Journal of Bacteriology·Céline RaynaudPhilippe Soucaille

Related Concepts

Glycerol dehydrogenase
Syntomycin
Molecular Sieve Chromatography
SDS-PAGE
Isoelectric Point
Klebsiella rhinoscleromatis
Dioxygen
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