In vivo induction of mucosal immune responses by intranasal administration of chitosan microspheres containing Bordetella bronchiseptica DNT

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Mi Lan KangHan Sang Yoo

Abstract

In vitro immune-stimulating activities of Bordetella bronchiseptica dermonecrotoxin (BBD)-loaded in chitosan microspheres (CMs) were reported with a mouse alveolar macrophage cell line (RAW264.7). Based on the report, in vivo activity of immune-induction was investigated by intranasal administration of the BBD-loaded CMs into mice. BBD was loaded into the CMs prepared by an ionic gelation process with tripolyphosphate. Mice were immunized by direct administration of the BBD-loaded CMs into the nasal cavity. After immunization of the mice, BBD-specific immune responses (IgG and IgA titers) were measured in sera, nasal wash, and saliva by ELISA. BBD-specific IgA titers in the nasal cavity were time- and dose-dependently increased by the administration. Similar phenomena were observed in the analysis of systemic IgA and IgG in sera. However, the antibody in saliva was undetectable by ELISA. These results suggested that direct vaccination via the nasal cavity was effective for targeting nasal-associated lymphoid tissues, and that CMs were an efficient adjuvant in nasal mucosal immunity for atrophic rhinitis vaccine.

References

Jan 1, 1990·Biomaterials, Artificial Cells, and Artificial Organs·T Chandy, C P Sharma
Feb 1, 1982·Journal of Medical Microbiology·J M RutterB F Sansom
Nov 24, 1999·Immunological Reviews·C CzerkinskyJ B Sun
Aug 23, 2001·Advanced Drug Delivery Reviews·L IllumS S Davis
Apr 1, 2005·Journal of Microencapsulation·J VarshosazR Alinagari
Apr 7, 2005·Nature Medicine·Jan Holmgren, Cecil Czerkinsky

❮ Previous
Next ❯

Citations

Aug 25, 2011·The Journal of Biological Chemistry·Chelsea L BueterStuart M Levitz
Dec 29, 2012·International Journal of Nanomedicine·Mohammad Ariful IslamChong-Su Cho
May 23, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kuntal GangulyTejraj M Aminabhavi
Jun 23, 2009·Expert Review of Vaccines·H Ciğdem ArcaSevda Senel
Jan 4, 2008·Journal of Drug Targeting·Bram SlütterWim Jiskoot
Nov 5, 2010·Journal of Drug Targeting·Inderjit Jabbal-Gill
Jun 26, 2014·Expert Opinion on Drug Delivery·Peggy RieseCarlos A Guzmán
May 11, 2007·Expert Opinion on Drug Delivery·Jaleh Varshosaz
Jun 23, 2015·Molecular Medicine Reports·Yanfeng GongNanjin Zhou
Jun 5, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Olga Kammona, Costas Kiparissides
Aug 31, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Merve GünbeyazSevda Senel
Jul 9, 2009·Biotechnology Advances·Mi Lan KangHan Sang Yoo
May 12, 2009·International Journal of Pharmaceutics·N BrandhonneurP Le Corre
Sep 20, 2008·International Immunopharmacology·Olga BorgesAnabela Cordeiro-da-Silva
Mar 28, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Olga BorgesHans E Junginger
Dec 3, 2020·Marine Drugs·Soojin Shim, Han Sang Yoo
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Sue D XiangMagdalena Plebanski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.