In vivo interference of paromomycin with mitochondrial activity of Leishmania

Experimental Cell Research
M MaaroufM Robert-Gero

Abstract

Paromomycin is an aminocyclitol aminoglycoside antibiotic used for the treatment of leishmaniasis. In view of the central role of mitochondria in cellular energetics and metabolism, its effect on in vivo mitochondrial activities of Leishmania donovani promastigotes-the parasite flagellate form-was investigated. The approach used flow cytometry, amperometric measure of O2 consumption, and, as a global estimate of mitochondrial dehydrogenases, thiazolyl blue reduction (MTT test); some in vitro controls were also made. When added to promastigote cultures for 24-72 h at 150-200 microM (= LC50), paromomycin doubled the generation time, inhibited respiration, and lowered its associated electric potential difference across mitochondrial membranes, as measured by rhodamine 123 fluorescence. The chemical analogue neomycin was ineffective. Furthermore, the in vivo mitochondrial dehydrogenase activities were lower, seemingly because of the shortage of respiratory substrates. Indeed, succinate addition to paromomycin-treated cultures partly restored mitochondrial membrane potential. However, no immediate effect of paromomycin on respiration was observed, neither inhibition of redox chain nor increase of membrane permeability (uncoupling). ...Continue Reading

References

Jul 1, 1975·FEBS Letters·M Miko, B Chance
Nov 1, 1992·Transactions of the Royal Society of Tropical Medicine and Hygiene·J A ScottA D Bryceson
Aug 1, 1992·Journal of the American Academy of Dermatology·J el-OnL Weinrauch
Dec 1, 1991·Molecular & General Genetics : MGG·O Ozier-KalogeropoulosF Lacroute
Sep 1, 1989·Molecular and Biochemical Parasitology·D ZilbersteinA Gepstein
May 1, 1989·Molecular and Biochemical Parasitology·R KiatfuengfooY Yuthavong
Jan 1, 1988·Annual Review of Cell Biology·L B Chen
Aug 1, 1985·The Journal of protozoology·A A DivoH Ginsburg
Apr 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D P EvensonM R Melamed
Mar 29, 1985·Biochemical and Biophysical Research Communications·S Abou-KhalilT J Lampidis
Feb 14, 1984·Biochemical and Biophysical Research Communications·J S Modica-NapolitanoJ R Aprille
Nov 1, 1983·Antimicrobial Agents and Chemotherapy·N TanakaT Nishimura
May 15, 1984·Archives of Biochemistry and Biophysics·A PeñaM Borbolla
Jul 1, 1984·Antimicrobial Agents and Chemotherapy·D C Eustice, J M Wilhelm
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·L B ChenT J Lampidis
Jan 1, 1995·Parasitology Research·M MaaroufM Robert-Gero
May 1, 1993·The Journal of Eukaryotic Microbiology·A A DivoA C Sartorelli
Jan 8, 1996·FEBS Letters·C RichterC Franceschi
Feb 1, 1996·Archives of Biochemistry and Biophysics·S N Moreno
Jan 1, 1997·Parasitology Research·M MaaroufM Robert-Gero

❮ Previous
Next ❯

Citations

Nov 15, 2006·Antimicrobial Agents and Chemotherapy·Sushma SinghRentala Madhubala
Apr 17, 2001·Antimicrobial Agents and Chemotherapy·J C DelorenziE M Saraiva
Dec 27, 2005·Antimicrobial Agents and Chemotherapy·Karin Seifert, Simon L Croft
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Jan 19, 2006·Clinical Microbiology Reviews·Simon L CroftAlan H Fairlamb
Jan 4, 2013·PLoS Neglected Tropical Diseases·Raquel García-HernándezFrancisco Gamarro
Jul 8, 2010·Journal of Global Infectious Diseases·Jaya Chakravarty, Shyam Sundar
Mar 6, 2010·Expert Opinion on Drug Delivery·Swati GuptaSuresh P Vyas
Jan 21, 2017·Parasitology·Silvia R B UlianaAdriano C Coelho
Oct 13, 2005·Molecular and Biochemical Parasitology·Angana MukherjeeRentala Madhubala
Feb 6, 2017·Bioorganic & Medicinal Chemistry·Alane B VermelhoClaudiu T Supuran
Jun 26, 2017·Drug Discovery Today·Bilal ZulfiqarVicky M Avery
Nov 19, 2017·Nature Communications·Moran Shalev-BenamiGeorgios Skiniotis
Aug 27, 2004·The Journal of Antimicrobial Chemotherapy·Ricardo M Santa-RitaSolange L de Castro
Dec 10, 2014·Antimicrobial Agents and Chemotherapy·María Ángeles AbengózarLuis Rivas
Oct 29, 2009·Pharmazie in unserer Zeit·Jasmin Ryczak, Conrad Kunick
Aug 2, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Patrice Le Pape
Oct 13, 2016·Cellular Immunology·Zahra AbdossamadiSima Rafati
May 1, 1999·Antimicrobial Agents and Chemotherapy·E C Torres-SantosB Rossi-Bergmann
Apr 8, 2021·Parasitology Research·Santanu Sasidharan, Prakash Saudagar
May 18, 2021·Frontiers in Cellular and Infection Microbiology·Elvira Cynthia Alves HorácioLaila Alves Nahum
May 15, 2004·Research in Microbiology·Luciana FumarolaOlga Brandonisio
Jun 26, 2021·European Journal of Medicinal Chemistry·Ojasvi GuptaVikramdeep Monga
Aug 15, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·B PapadopoulouM Ouellette
Sep 26, 2014·Chemical Reviews·Hidayat HussainSimon Gibbons

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.