In vivo intermembrane transfer of phospholipids in the photosynthetic bacterium Rhodopseudomonas sphaeroides.

Journal of Bacteriology
B D CainS Kaplan

Abstract

The kinetics of accumulation of phospholipids into the intracytoplasmic membrane of Rhodopseudomonas sphaeroides have been examined. We have previously demonstrated that accumulation of phospholipids in the intracytoplasmic membrane is discontinuous with respect to the cell cycle. In this study we demonstrated a sevenfold increase in the rate of phospholipid incorporation into the intracytoplasmic membrane concurrent with the onset of cell division. Pulse-chase labeling studies revealed that the increase in the rate of phospholipid accumulation into the intracytoplasmic membrane results from the transfer of phospholipid from a site other than the intracytoplasmic membrane, and that the transfer of phospholipid, rather than synthesis of phospholipid, is most likely subject to cell cycle-specific regulation. The rates of synthesis of the individual phospholipid species (phosphatidylethanolamine, phosphatidyglycerol, and an unknown phospholipid) remained constant with respect to one another throughout the cell cycle. Similarly, each of these phospholipid species appeared to be transferred simultaneously to the intracytoplasmic membrane. We also present preliminary kinetic evidence which suggested that phosphatidylethanolamine may ...Continue Reading

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Citations

Jan 1, 1983·Journal of Cellular Biochemistry·S KaplanG S Yen
May 25, 2012·Molecular Microbiology·Philip J JacksonMark J Dickman
Jun 15, 1990·Experientia·G Daum, F Paltauf
Feb 1, 1983·Journal of Bacteriology·T B Campbell, D R Lueking
Nov 1, 1982·Journal of Bacteriology·F E Nano, S Kaplan
Aug 31, 1984·Biochimica Et Biophysica Acta·M Snozzi, A R Crofts
Jul 24, 1986·Biochimica Et Biophysica Acta·S P TaiS Kaplan
Mar 26, 1987·Biochimica Et Biophysica Acta·J H HogerS Kaplan
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