In vivo internalization of the somatostatin sst2A receptor in rat brain: evidence for translocation of cell-surface receptors into the endosomal recycling pathway

Molecular and Cellular Neurosciences
Zsolt CsabaP Dournaud

Abstract

To determine whether cellular compartmentalization of somatostatin receptors can be regulated in vivo, we examined the immunocytochemical distribution of the sst2A receptor (sst2AR) after stereotaxical injections of somatostatin analogs into the rat parietal cortex. Whereas CH-275, a sst1R agonist, failed to induce changes in the diffuse sst2AR immunostaining pattern characteristic of control animals, somatodendritic profiles displaying intracytoplasmic immunoreactive granules became apparent short-term after injection of either somatostatin or the sst2R agonist octreotide. Confocal microscopy revealed that 90% of sst2AR-immunoreactive endosome-like organelles displayed transferrin receptor immunoreactivity. At the electron microscopic level, the percentage of sst2AR immunoparticles dramatically decreased at the plasmalemma of perikarya and dendrites after octreotide injection. Conversely, it significantly increased in endosomes-like organelles. These results demonstrate that sst2ARs undergo, in vivo, rapid and massive internalization into the endocytic recycling compartment in response to acute agonist stimulation and provide important clues toward elucidating somatostatin receptor signaling in the mammalian brain.

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Citations

Dec 14, 2011·Journal of Molecular Endocrinology·Zsolt CsabaPascal Dournaud
Apr 7, 2007·European Journal of Endocrinology·Giovanni Tulipano, Stefan Schulz
Nov 30, 2007·Molecular and Cellular Endocrinology·Stefan Jacobs, Stefan Schulz
Jan 31, 2006·Trends in Neurosciences·Véronique BernardBertrand Bloch
Nov 1, 2011·Progress in Neurobiology·Xueyi Li, Marian DiFiglia
May 11, 2001·Neuropeptides·Z Csaba, P Dournaud
Mar 12, 2013·Neurobiology of Disease·Maxim KozhemyakinJaideep Kapur
Dec 5, 2003·Nature Reviews. Drug Discovery·Gisbert WeckbeckerChristian Bruns
Oct 29, 2005·Journal of Neuropathology and Experimental Neurology·Zsolt CsabaPascal Dournaud
Sep 9, 2020·Neuropathology and Applied Neurobiology·Z CsabaP Dournaud
Feb 26, 2005·Journal of Cell Science·Fabien PujolHélène Boudin
Apr 12, 2002·Progress in Neurobiology·A Merighi

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