In vivo investigation of the transcription, processing, endonucleolytic activity, and functional relevance of the spatial distribution of a plant miRNA

Genes & Development
Eneida Abreu ParizottoOlivier Voinnet

Abstract

We show, with miR171, that plant miRNA genes are modular independent transcription units in which the fold-back pre-miRNA is sufficient for miRNA processing, and that the upstream region contains highly specific promoter elements. Processing depends on flanking sequences within the miRNA stem-loop precursor rather than the miRNA sequence itself, and mutations affecting target pairing at the center and 5' but not 3' region of the miRNA compromise its function in vivo. Inactivation of the SDE1 RNA-dependent-RNA-polymerase was mandatory for accurate representation of miRNA activity by sensor constructs in Arabidopsis. Work in sde1 background revealed a near-perfect spatial overlap between the patterns of miR171 transcription and activity, supporting the idea that plant miRNAs enable cell differentiation.

References

May 26, 1999·The Plant Journal : for Cell and Molecular Biology·L D PyshP N Benfey
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Jul 9, 2002·Genes & Development·Brenda J ReinhartDavid P Bartel
Aug 3, 2002·Science·György Hutvágner, Phillip D Zamore
Aug 31, 2002·Cell·Matthew W RhoadesDavid P Bartel
Jan 7, 2003·Genes & Development·Guiliang TangPhillip D Zamore
Feb 15, 2003·Developmental Cell·Kristin D KasschauJames C Carrington
Feb 26, 2003·Genes & Development·John G DoenchPhillip A Sharp
Jun 14, 2003·Plant Physiology·Bonnie Bartel, David P Bartel
Aug 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yan ZengBryan R Cullen
Aug 22, 2003·Nature·Javier F PalatnikDetlef Weigel
Aug 28, 2003·The EMBO Journal·Christophe HimberOlivier Voinnet
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Oct 7, 2003·Nature Genetics·Emily BernsteinGregory J Hannon
Mar 6, 2004·Nature·Catherine A Kidner, Robert A Martienssen
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp
Apr 24, 2004·Science·Soraya YektaDavid P Bartel
Jun 1, 2004·Nature Structural & Molecular Biology·Benjamin Haley, Phillip D Zamore

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Citations

Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·William M StraussDana Ridzon
Jul 5, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Caifu ChenWilliam M Strauss
Nov 3, 2010·Protoplasma·Tae Kyung HyunJae-Yean Kim
May 6, 2005·Functional & Integrative Genomics·Anthony A Millar, Peter M Waterhouse
Mar 23, 2012·Biotechnology Letters·Xuming WangJianping Chen
Jun 10, 2011·Molecular and Cellular Biochemistry·Yunzhi ZhangShilin Li
Oct 20, 2011·Plant Molecular Biology·Yingyin Yao, Qixin Sun
Dec 14, 2011·Plant Molecular Biology·Ying-Hsuan SunRonald R Sederoff
May 25, 2010·Molecular Biotechnology·Xuming WangJianping Chen
Apr 16, 2005·Current Opinion in Biotechnology·Christophe Ritzenthaler
Feb 4, 2005·Nature·Michael Ronemus, Rob Martienssen
Apr 23, 2010·Nature·Annelie CarlsbeckerPhilip N Benfey
Nov 27, 2004·Nature Genetics·Olivier Voinnet
Mar 1, 2012·Nature Protocols·Marie Javelle, Marja C P Timmermans
Jan 16, 2009·Nature Reviews. Molecular Cell Biology·Peter Brodersen, Olivier Voinnet
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Cinzia SevignaniLinda D Siracusa
Jan 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Peter BrodersenOlivier Voinnet
Jan 24, 2008·Human Gene Therapy·Gregory Ku, Michael T McManus
Dec 8, 2010·Nucleic Acids Research·Felipe Fenselau de FelippesDetlef Weigel
Feb 4, 2009·Plant & Cell Physiology·Shunsuke MiyashimaKeiji Nakajima
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Alban JacquesSerge Kauffmann
Apr 27, 2013·Phytopathology·Fernando MartínezJosé-Antonio Daròs
Mar 10, 2009·Genes & Development·Daniel H ChitwoodMarja C P Timmermans
Aug 9, 2008·Genome Research·Qian-Hao ZhuChris Helliwell
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·F T S NogueiraM C P Timmermans
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·D C Baulcombe
Jun 16, 2005·Plant Physiology·James C Carrington
Jul 26, 2005·Plant Physiology·Zhixin XieJames C Carrington
Jun 9, 2007·Plant Physiology·Craig Anthony Atkins, Penelope Mary Collina Smith
Apr 30, 2008·Plant Physiology·Elsa M TretterJohn L Bowman
Apr 5, 2005·The Plant Cell·Ramanjulu SunkarJian-Kang Zhu
Apr 26, 2005·The Plant Cell·Michael J Axtell, David P Bartel
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel

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