In vivo investigations of the effect of short- and long-term recombinant growth hormone treatment on DNA-methylation in humans

PloS One
Julia KolarovaSusanne Bens

Abstract

Treatment with recombinant human growth hormone (rhGH) has been consistently reported to induce transcriptional changes in various human tissues including peripheral blood. For other hormones it has been shown that the induction of such transcriptional effects is conferred or at least accompanied by DNA-methylation changes. To analyse effects of short term rhGH treatment on the DNA-methylome we investigated a total of 24 patients at baseline and after 4-day rhGH stimulation. We performed array-based DNA-methylation profiling of paired peripheral blood mononuclear cell samples followed by targeted validation using bisulfite pyrosequencing. Unsupervised analysis of DNA-methylation in this short-term treated cohort revealed clustering according to individuals rather than treatment. Supervised analysis identified 239 CpGs as significantly differentially methylated between baseline and rhGH-stimulated samples (p<0.0001, unadjusted paired t-test), which nevertheless did not retain significance after adjustment for multiple testing. An individualized evaluation strategy led to the identification of 2350 CpG and 3 CpH sites showing methylation differences of at least 10% in more than 2 of the 24 analyzed sample pairs. To investigate th...Continue Reading

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Citations

Mar 14, 2021·Reviews in Endocrine & Metabolic Disorders·Adam StevensPhilip Murray
May 29, 2021·The Pharmacogenomics Journal·Adam StevensPeter Clayton

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Datasets Mentioned

BETA
GM12878

Methods Mentioned

BETA
SGA
Assay
PCR
electrophoresis
PCA
acetylation
ChIP-Seq

Software Mentioned

GenomeStudio
Ensembl
Gene Annotation Tool to Help Explain Relationships GATHER
PyroMark Assay Design
PyroMark CpG
PyroMark
Gene Annotation Tool to GATHER
R
Omics Explorer

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