PMID: 9188279Jun 1, 1997Paper

In vivo laser-induced fluorescence imaging of a rat pancreatic cancer with pheophorbide-a

Photochemistry and Photobiology
V TassettiMarc Aprahamian

Abstract

Laser-induced fluorescence (LIF) of pheophorbide-a (Ph-a) was used for imaging of a rat pancreatic tumor. Using a dimensionless function (the ratio of Ph-a fluorescence by bluish autofluorescence), the fluorescence contrasts between excised tumors and their paired pancreas were investigated up to 48 h after a 9 mg kg-1 Ph-a intravenous administration. Among five tested excitation wavelengths, 355 and 610 nm excitations gave the best distinctive contrasts, both 48 h after dye injection. The LIF imaging of six intrapancreatic tumors and six healthy pancreas was carried out in vivo using two laser excitations: 355 nm (Nd:YAG + tripling) for bluish autofluorescence and 610 nm (rhodamine 6G dye) for reddish autofluorescence and dye emission. Images were recorded through bandpass filters at 470 and 640 nm (autofluorescence) and at 680 nm (dye + autofluorescence) with an intensified charged-coupled device camera. Autofluorescence as Ph-a fluorescence images did not allow accurate LIF diagnosis of pancreatic carcinoma. An image processing, including for each pixel a computed division of Ph-a fluorescence (after subtraction of reddish autofluorescence) by bluish autofluorescence intensity generated poorly contrasted tumor images in five...Continue Reading

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Citations

Jul 27, 1999·The British Journal of Surgery·A HajriM Aprahamian
Aug 12, 2014·Photomedicine and Laser Surgery·Yu NagaiAkiyoshi Saito
Jan 6, 2017·Organic & Biomolecular Chemistry·Anjul KhadriaHarry L Anderson
Oct 25, 2006·Applied Spectroscopy·Moon S KimMoonjohn Kim
Feb 8, 2000·Physics in Medicine and Biology·T Schunck, P Poulet
Jul 23, 2003·Journal of Food Protection·Moon S KimYud-Ren Chen

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