PMID: 9428599Jan 15, 1998Paper

In vivo localization of diglycylcysteine-bearing synthetic peptides by nuclear imaging of oxotechnetate transchelation

Nuclear Medicine and Biology
A BogdanovR Weissleder

Abstract

A phenomenon of in vivo transchelation of oxotechnetate from a complex with glucoheptonic acid to synthetic peptides bearing oxotechnetate-binding motifs and a technique for in vivo visualization of these peptides are described. Using two model peptides bearing two tandem diglycylcysteine (GGC) motifs (P1) or three GGC motifs (P2), we demonstrated that: (i) these peptides efficiently transchelated oxo-[99mTc]technetate from a complex with glucoheptonic acid in vitro (a complex with peptides was stable at least 24 h; radiochemical purity exceeded 95% by high performance liquid chromatography); (ii) injection of peptides into the rectus femoris muscle (at 0.5-1 micromol of SH groups) followed by an intravenous injection of 99mTc-glucoheptonate (0.25-0.5 mCi per animal) yielded visualization of the injected muscle by nuclear imaging within 1 h after injection; (iii) the experimental/control (contralateral) thigh muscle ratio was 1.80 +/- 0.05 for peptide P1 and 3.0 +/- 0.1 for P2; (iv) the injection of a control peptide P2 with SH groups covalently modified with N-ethylmaleimide resulted in a ratio of 1.4 +/- 0.2. These findings argue for specific association of oxo-[99mTc]technetate with free thiols within the binding motif of in...Continue Reading

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Citations

Feb 3, 2000·Annals of Nuclear Medicine·U Haberkorn
Apr 18, 1998·Biochimica Et Biophysica Acta·A BogdanovR Weissleder
Nov 17, 2001·Seminars in Nuclear Medicine·P RayS S Gambhir
Apr 27, 2001·Radiology·R Weissleder, U Mahmood
Apr 12, 2003·Neuroimaging Clinics of North America·Dawid Schellingerhout, Alexei A Bogdanov
Jan 7, 2004·Clinical Journal of Oncology Nursing·Scott Olszewski

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