In vivo localization of iris yellow spot tospovirus (Bunyaviridae)-encoded proteins and identification of interacting regions of nucleocapsid and movement proteins

PloS One
Diwaker TripathiHanu R Pappu

Abstract

Localization and interaction studies of viral proteins provide important information about their replication in their host plants. Tospoviruses (Family Bunyaviridae) are economically important viruses affecting numerous field and horticultural crops. Iris yellow spot virus (IYSV), one of the tospoviruses, has recently emerged as an important viral pathogen of Allium spp. in many parts of the world. We studied the in vivo localization and interaction patterns of the IYSV proteins in uninfected and infected Nicotiana benthamiana and identified the interacting partners. Bimolecular fluorescence complementation (BiFC) analysis demonstrated homotypic and heterotypic interactions between IYSV nucleocapsid (N) and movement (NSm) proteins. These interactions were further confirmed by pull-down assays. Additionally, interacting regions of IYSV N and NSm were identified by the yeast-2-hybrid system and β-galactosidase assay. The N protein self-association was found to be mediated through the N- and C-terminal regions making head to tail interaction. Self-interaction of IYSV NSm was shown to occur through multiple interacting regions. In yeast-2-hybrid assay, the N- and C-terminal regions of IYSV N protein interacted with an N-terminal re...Continue Reading

References

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Citations

Mar 11, 2017·Virus Research·Sung-Hwan KangSvetlana Y Folimonova
May 24, 2019·Journal of Virology·Xin ZhouZhenghe Li
Jun 15, 2016·Annual Review of Phytopathology·Massimo TurinaRenato O Resende
Sep 1, 2016·Annual Review of Virology·J E Oliver, A E Whitfield
Apr 27, 2017·Frontiers in Microbiology·Shirani M K Widana Gamage, Ralf G Dietzgen
Jun 3, 2021·Viruses·Richard KormelinkCecile Desbiez

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Datasets Mentioned

BETA
/AF214014.1

Methods Mentioned

BETA
bimolecular fluorescence complementation
pull down
transgenic
PCR
pull-down
two-hybrid

Software Mentioned

INSV
BiFC

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