In vivo localized 1H MR spectroscopy of rat testes: stimulated echo acquisition mode (STEAM) combined with short TI inversion recovery (STIR) improves the detection of metabolite signals

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Masayuki YamaguchiManabu Minami

Abstract

A noninvasive NMR technique for evaluating testicular function was explored in this study. Localized in vivo 1H NMR spectroscopy was performed on rat testes using a stimulated echo acquisition mode (STEAM) sequence with a short echo time (TE). In the 1H spectra, large lipid signals dominated the chemical shift range of 0.89-2.78 ppm, which prevented the observation of metabolite signals in this region. To suppress these lipid signals, short inversion time (TI) inversion recovery (STIR) was combined with STEAM (STIR-STEAM). The optimal TI was typically 320 ms. STIR-STEAM with a TE of 15 ms allowed successful suppression of the lipid signals and the sensitive detection of several new metabolite signals. In normal testes, choline, creatine, glutamate, and glycine signals were identified. In addition to these metabolites, a lactate signal was observed in ischemic testes. To our knowledge, the signals of glutamate, glycine, and lactate have not been previously assigned in 1H MR spectra of testes in vivo. Lipid suppression by STIR aided in the detection of these metabolites, which would otherwise have been masked by the lipid signals.

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Citations

Nov 1, 2007·International Journal of Andrology·C MallidisR A Browne
Oct 18, 2015·European Radiology·Athina C TsiliMaria I Argyropoulou
Aug 31, 2007·Magnetic Resonance Imaging·Ahmet Kemal FiratSelim Doğanay
Jan 19, 2018·Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine·Masayuki Yamaguchi, Hirofumi Fujii
Feb 17, 2018·Insights Into Imaging·Gian Carlo ParentiMelchiore Giganti
Oct 29, 2019·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jan MartinFrederik Bernd Laun
Dec 19, 2020·Journal of Magnetic Resonance Imaging : JMRI·Yusuke TakahashiYasushi Sakata

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