In vivo low-density lipoprotein exposure induces intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 correlated with activator protein-1 expression

Arteriosclerosis, Thrombosis, and Vascular Biology
Lynne VernaMichael B Stemerman

Abstract

We tested the hypothesis that direct native low-density lipoprotein (LDL) injection into LDL receptor-deficient (LDLR(-/-)) mice would induce the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in their aortic endothelial cells, and that transcriptional regulation of this pathway involved activator protein-1 (AP-1) but not nuclear factor kappaB (NF-kappaB). Using tail vein injection of LDL into LDLR(-/-) mice, we were able to maintain atherogenic LDL blood levels, which induced ICAM-1 and VCAM-1 expression in their aortic endothelial cells after 24 hours. We were able to visualize and quantify this expression using immunohistochemistry and confocal microscopy. Under conditions in which ICAM-1 and VCAM-1 were expressed, the regulatory AP-1 proteins c-Fos and c-Jun were also highly expressed in the endothelial cell cytoplasm and observed within the cell nucleus. The NF-kappaB protein P65, although expressed in the endothelial cell cytoplasm after LDL injection, was not observed within the cell nucleus. Elevated LDL blood levels, maintained in vivo, increased the expression of the adhesion molecules ICAM-1 and VCAM-1 in aortic endothelial cells. This effect appeared to c...Continue Reading

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Citations

Jan 7, 2009·Cardiovascular Drugs and Therapy·Carlos V SerranoHugo P Monteiro
Feb 6, 2014·The International Journal of Biochemistry & Cell Biology·Qing-Ping YaoZong-Lai Jiang
Jan 16, 2007·Current Opinion in Lipidology·Marie Goua, Klaus Wj Wahle
Mar 17, 2009·Circulation Research·Cornelia HahnMartin Alexander Schwartz
Aug 20, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Olivier CalvayracJosé Martínez-González
Aug 4, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Elena Galkina, Klaus Ley
Oct 25, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Hideto YamadaYoshihiro Ogawa
Dec 25, 2008·Environmental and Molecular Mutagenesis·Haw-Wen ChenTsu-Shing Wang
Mar 17, 2018·Current Medicinal Chemistry·Johan G SchnitzlerJeffrey Kroon
Aug 5, 2017·Journal of Cardiovascular Pharmacology·Thomas P JohnstonAmirhossein Sahebkar
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chaojie ZhuQiwen Wang
May 6, 2008·Toxicology Letters·Elizabeth OesterlingBernhard Hennig
Feb 24, 2009·Medicina clínica·María Matilde Ramírez AlvaradoTibisay Pelayo Jordán

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