Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.
Femtosecond near-infrared lasers as a novel tool for non-invasive real-time high-resolution time-lapse imaging of chloroplast division in living bundle sheath cells of Arabidopsis
Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis
Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope
Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics
Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator
Chromophore-assisted laser inactivation--towards a spatiotemporal-functional analysis of proteins, and the ablation of chromatin, organelle and cell function
Cell Imaging in CNS
Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.