In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation

Journal of Biomedical Optics
Wataru WatanabeKazuyoshi Itoh

Abstract

Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.

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Citations

Jan 13, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nanhong LouMaiken Nedergaard
Oct 23, 2012·Journal of Bioscience and Bioengineering·Tsunehito HigashiSachihiro Matsunaga
Feb 4, 2009·Optics Express·Yuan ZhaoShaoqun Zeng
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Jun 15, 2010·Analytical Chemistry·Aleeta M PoweIsiah M Warner

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