In vivo mapping of tissue- and subcellular-specific proteomes in Caenorhabditis elegans

Science Advances
Aaron W ReinkeEric J Bennett

Abstract

Multicellular organisms are composed of tissues that have distinct functions requiring specialized proteomes. To define the proteome of a live animal with tissue and subcellular resolution, we adapted a localized proteomics technology for use in the multicellular model organism Caenorhabditis elegans. This approach couples tissue- and location-specific expression of the enzyme ascorbate peroxidase (APX), which enables proximity-based protein labeling in vivo, and quantitative proteomics to identify tissue- and subcellular-restricted proteomes. We identified and localized more than 3000 proteins from strains of C. elegans expressing APX in either the nucleus or cytoplasm of the intestine, epidermis, body wall muscle, or pharyngeal muscle. We also identified several hundred proteins that were specifically localized to one of the four tissues analyzed or specifically localized to the cytoplasm or the nucleus. This approach resulted in the identification both of proteins with previously characterized localizations and of those not known to localize to the nucleus or cytoplasm. Further, we confirmed the tissue- and subcellular-specific localization of a subset of identified proteins using green fluorescent protein tagging and fluore...Continue Reading

References

May 10, 2002·Nucleic Acids Research·David M Hoover, Jacek Lubkowski
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jul 7, 2005·Genome Research·C Victor JongeneelThomas J Vasicek
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Apr 9, 2008·Developmental Biology·Frederick A PartridgeJonathan Hodgkin
Oct 28, 2008·Nature Genetics·Christian Frøkjaer-JensenErik M Jorgensen
Mar 21, 2009·Nature Protocols·Paul J BoersemaAlbert J R Heck
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John N WernerZemer Gitai
Aug 12, 2009·Proteomics·Jing LiFuquan Yang
Dec 8, 2009·Nucleic Acids Research·Siew Loon OoiSteven Henikoff
Dec 24, 2010·Genome Research·W Clay SpencerDavid M Miller
Feb 1, 2012·Nature Methods·Christian Frøkjær-JensenErik M Jorgensen
Mar 14, 2012·Nature Reviews. Genetics·Christine Vogel, Edward M Marcotte
Feb 26, 2013·Molecular & Cellular Proteomics : MCP·Tamar GeigerMatthias Mann
Mar 20, 2013·The Journal of Cell Biology·Michal BrekerMaya Schuldiner
Jul 23, 2013·Nature Protocols·Huaiyu MiPaul D Thomas
Nov 5, 2013·Molecular & Cellular Proteomics : MCP·Jesper V Olsen, Matthias Mann
May 30, 2014·Nature·Min-Sik KimAkhilesh Pandey
Nov 25, 2014·Nature Methods·Stephanie S LamAlice Y Ting
Feb 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kai P YuetDavid A Tirrell
Sep 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chiao-Lin ChenNorbert Perrimon
Oct 7, 2015·Current Biology : CB·Martin WührSteven P Gygi

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Citations

Aug 21, 2018·Nature Biotechnology·Tess C BranonAlice Y Ting
Sep 11, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Justin A BoschNorbert Perrimon
Jul 22, 2018·Proteomes·Yi-Zhi Wang, Jeffrey N Savas
Jul 19, 2017·Proteomes·Evelyn Ramberger, Gunnar Dittmar
Mar 16, 2018·Molecular Biology of the Cell·Nathan ZuzowEric J Bennett
Jun 19, 2019·Development·Katelynn M MannixLynn Cooley
Dec 23, 2020·Journal of Neuroendocrinology·Sara Van DammeIsabel Beets
Nov 14, 2019·Biochemistry·Thanh My Thi NguyenMihye Lee
Feb 16, 2021·G3 : Genes - Genomes - Genetics·Dustin Haskell, Anna Zinovyeva
Aug 18, 2020·Current Opinion in Chemical Biology·Ying Zhou, Peng Zou
Nov 11, 2017·Current Opinion in Neurobiology·Shuo HanAlice Y Ting
May 7, 2021·MSphere·Aaron W Reinke
Aug 21, 2021·The Journal of Biological Chemistry·Murat ArtanMario de Bono

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Methods Mentioned

BETA
transgenic
dissection
fluorescence microscopy
electrophoresis
Protein Assay
reversed-phase

Software Mentioned

PANTHER
exe
ReAdW
SEQUEST

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