In Vivo Metabolic Tracing Demonstrates the Site-Specific Contribution of Hepatic Ethanol Metabolism to Histone Acetylation

Alcoholism, Clinical and Experimental Research
Crystina L KrissStanley M Stevens

Abstract

Epigenetic dysregulation through ethanol (EtOH)-induced changes in DNA methylation and histone modifications has been implicated in several alcohol-related disorders such as alcoholic liver disease. EtOH metabolism in the liver results in the formation of acetate, a metabolite that can be converted to acetyl-CoA, which can then be used by histone acetyltransferases to acetylate lysine residues. EtOH metabolism in the liver can also indirectly influence lysine acetylation through NAD+ -dependent sirtuin activity that is altered due to increases in NADH. As a proof-of-concept study to determine the direct influence of hepatic EtOH metabolism on histone acetylation changes, we used heavy-labeled EtOH (13 C2 ) and mass spectrometry (MS) to site specifically characterize lysine acetylation on histone proteins. Eight-week-old male C57BL/6J mice were gavaged using a bolus dose of either 13 C2 -labeled EtOH (5 g/kg) or maltose dextrin. Blood and livers were collected at 0, 4, and 24 hours followed by histone protein enrichment and derivatization using acid extraction and propionylation, respectively. Metabolic tracing and relative quantitation of acetylated histone proteins were performed using a hybrid quadrupole-orbitrap mass spectro...Continue Reading

References

Mar 6, 1998·Cellular and Molecular Life Sciences : CMLS·B M Turner
Jun 14, 2003·Biochemical and Biophysical Research Communications·Pil-Hoon ParkShivendra D Shukla
Mar 12, 2004·Biochemical and Biophysical Research Communications·K ItoI M Adcock
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pil-Hoon ParkShivendra D Shukla
Feb 2, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Min YouGene C Ness
Mar 14, 2008·Alcoholism, Clinical and Experimental Research·Mahua Choudhury, Shivendra D Shukla
Jun 17, 2008·Biochemical and Biophysical Research Communications·Charles S LieberLeonore M Decarli
Jul 12, 2008·Alcoholism, Clinical and Experimental Research·Shivendra D ShuklaSamir Zakhari
Sep 23, 2008·Biochemical and Biophysical Research Communications·Matthew J Picklo
Sep 23, 2008·The International Journal of Biochemistry & Cell Biology·Stephanie SpangeOliver H Krämer
Nov 11, 2008·Human Molecular Genetics·Claire L SimpsonAmmar Al-Chalabi
Mar 18, 2009·World Journal of Gastroenterology : WJG·Blythe D Shepard, Pamela L Tuma
Dec 3, 2009·Alcoholism, Clinical and Experimental Research·Blythe D ShepardPamela L Tuma
Mar 17, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stuart F W KendrickChris P Day
Aug 19, 2010·Molecular & Cellular Proteomics : MCP·Lennart MartensEric W Deutsch
Sep 25, 2010·Bulletin of the World Health Organization·Thomas F Babor
Nov 16, 2010·Trends in Biochemical Sciences·Go-Woon Kim, Xiang-Jiao Yang
Dec 8, 2010·Epigenetics & Chromatin·Barry M ZeeBenjamin A Garcia
Mar 5, 2011·Nutrition & Metabolism·Jigneshkumar PatelShiraz Mujtaba
Jun 3, 2011·World Journal of Gastroenterology : WJG·Pranoti Mandrekar
Jul 5, 2011·Parasite Immunology·Y Adomako-AnkomahJ P Boyle
Oct 18, 2011·Expert Review of Proteomics·Laura-Mae P BrittonBenjamin A Garcia
Feb 4, 2012·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Pil-Hoon ParkShivendra D Shukla
May 2, 2012·Proteome Science·Annayya R AroorShivendra D Shukla
Jul 10, 2012·Cell Metabolism·Chao Lu, Craig B Thompson
Oct 12, 2012·Nature Biotechnology·Matthew C ChambersParag Mallick
Feb 6, 2013·Methods in Molecular Biology·Barry M Zee, Benjamin A Garcia
Mar 2, 2013·Nature Protocols·Adeline BertolaBin Gao
Mar 8, 2013·Cellular and Molecular Life Sciences : CMLS·Claudia Cosentino, Raul Mostoslavsky
Mar 14, 2013·The Journal of Biological Chemistry·Adam G EverttsBenjamin A Garcia
Aug 3, 2013·Alcoholism, Clinical and Experimental Research·Irina KirpichShirish Barve
Sep 10, 2013·Methods in Molecular Biology·Yu LiDavid B Lombard
Sep 28, 2013·PloS One·Kristofer S FritzMatthew D Hirschey
Jul 24, 2014·Nature Reviews. Molecular Cell Biology·Chunaram ChoudharyMatthias Mann
Aug 29, 2014·Journal of Proteome Research·Zuo-Fei YuanBenjamin A Garcia
Jan 1, 2013·Journal of Molecular Psychiatry·Vanessa NieratschkerAndreas J Fallgatter
Jan 1, 2015·Nature Reviews. Molecular Cell Biology·Eric Verdin, Melanie Ott
Apr 19, 2015·The Journal of Pharmacology and Experimental Therapeutics·Shivendra D ShuklaJamal A Ibdah
Jun 10, 2015·Biochemical and Biophysical Research Communications·Sun Ju KimSangkyu Lee
Nov 26, 2015·Epigenomics·Roberta NoberiniTiziana Bonaldi
Sep 2, 2017·Scientific Reports·Yekaterina KoriBenjamin A Garcia

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Citations

Mar 4, 2020·Expert Review of Gastroenterology & Hepatology·Liping ChenDesong Kong
Jul 23, 2020·Nature Metabolism·Alia GhrayebInbal Mor
Sep 11, 2020·Nature Reviews. Genetics·Ziwei DaiJason W Locasale
Mar 23, 2021·Current Pathobiology Reports·Russell S DulmanSubhash C Pandey

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