PMID: 2105748Feb 6, 1990Paper

In vivo metabolism of platelet-activating-factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by the protozoan Tetrahymena pyriformis

Biochimica Et Biophysica Acta
M E LekkaV M Kapoulas

Abstract

The metabolism of exogenous platelet-activating-factor was studied in the protozoan Tetrahymena pyriformis in vivo. When the cells are exposed to 1.10(-6) M PAF, the molecule is rapidly metabolized to 1-O-alkyl-2-acyl(long chain)-GPC, a major component of the protozoan membranes. The appearance of lyso-PAF from the first minutes even in low levels provides evidence that deacetylation is an intermediate step. After incubation for 30 min, transformation to aminoethyl phosphonolipids is also observed. The fate of PAF in concentrations 1.5.10(-11) M or 1.10(-8) M PAF, was the same. An amount of PAF depending on the external PAF concentration remained intact in the cell even after 1 h incubation. Our results suggest that the easily cultured protozoan can be a useful model for studying PAF's metabolism.

References

May 31, 1977·Biochimica Et Biophysica Acta·G A Thompson, Y Nozawa
Mar 4, 1969·Biochimica Et Biophysica Acta·V M Kapoulas
Apr 18, 1972·Biochimica Et Biophysica Acta·H BergerD J Hanahan
Mar 4, 1969·Biochimica Et Biophysica Acta·G A Thompson
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Aug 19, 1991·FEBS Letters·A D TselepisD Tsoukatos
Apr 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A KarkabounasM E Lekka
Mar 28, 1991·European Journal of Biochemistry·F BussolinoS Bozzaro
Oct 1, 1995·Protein Science : a Publication of the Protein Society·O H KappS N Vinogradov
Dec 3, 1999·Biochemical and Biophysical Research Communications·C O RodriguesA H Lopes

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