PMID: 8967791Apr 1, 1996Paper

In vivo microautoradiography of [3H]1,24(OH)2D3 (tacalcitol) following topical application to normal rats and in vitro metabolism in human keratinocytes

Archives of Dermatological Research
T OhtaM Kiyoki

Abstract

This study was conducted to investigate the mechanism of topical absorption of [3H]1,24(OH)2D3 (1,24-dihydroxyvitamin D3; tacalcitol) by applying an ointment containing 4 micrograms2/g [3H]1,24(OH)2D3 to the skin of rats using an occlusion method. Microautoradiography of the skin at the application site 1 h after topical treatment showed a high concentration of radiolabel in the stratum corneum, the epidermis and around the hair follicles. Radiolabel was also seen in the epidermis and hair follicle areas 8 h and 24 h after application. The radiolabel was distributed to a minor extent to the subcutaneous fat layer. Microautoradiography showed two routes of purcutaneous absorption of 1,24(OH)2D3: through the stratum corneum and epidermis into the microvessels, and through hair follicle areas into the bloodstream. After topical application of an ointment containing 4 micrograms/g or 40 micrograms/g [3H]1,24(OH)2D3 to the shaved neck skin of rats, the absorption rate, estimated by excretion in the urine and faeces, was about 30% of the total applied radioactivity. The main excretion route after topical application was in the faeces. Furthermore, 1,24(OH)2D3 added to human adult keratinocytes was not metabolized into other compounds...Continue Reading

References

Feb 1, 1990·The Journal of Dermatology·K MatsumotoK Yoshikawa
Sep 1, 1988·The Journal of Clinical Endocrinology and Metabolism·U BergerR C Coombes
Oct 1, 1986·The British Journal of Dermatology·S MorimotoY Kumahara
Jan 1, 1984·Cell and Tissue Research·W E StumpfH F DeLuca
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R U Simpson, H F DeLuca

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Citations

Jul 16, 2011·Clinical, Cosmetic and Investigational Dermatology·G Márquez BalbásP Umbert Millet
Nov 6, 2007·Experimental Dermatology·Walter E StumpfHans-Juergen Bidmon
Sep 24, 2004·Journal of Pharmacological and Toxicological Methods·Naohiko HayakawaWalter E Stumpf

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