In vivo microscopy in a porcine model of acute lung injury

Respiratory Physiology & Neurobiology
Johannes BickenbachRolf Rossaint

Abstract

Regional inhomogeneity and alveolar mechanics in a porcine model of acute lung injury (ALI) was evaluated using confocal laser scanning microscopy (CLSM). CLSM was performed through thoracic windows of the upper and lower lobes. Image quantification was conducted by use of a volume air index (VAI). Twelve anesthetized, mechanically ventilated pigs were randomized to non-injury (control group, n = 6) or ALI induced by surfactant depletion (ALI group, n = 6). CLSM was performed at baseline, after 1 h at 5 mbar and after 2 h at 15 mbar positive end-expiratory pressure (PEEP). Haemodynamics, respiratory mechanics and calculation of pulmonary ventilation-perfusion distribution by MIGET were determined. At baseline, VAI was not different. In the upper lobes, VAI significantly decreased in ALI compared to control group, with no changes after PEEP application. In the lower lobes, VAI significantly decreased in ALI compared to control group. Incremental PEEP significantly increased VAI in ALI, but not in control group. Haemodynamics were significantly compromised in the ALI group. A significant deterioration in oxygenation and ventilation-perfusion distribution could be seen being restored after PEEP adjustment. The VAI may help to asse...Continue Reading

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Citations

Aug 22, 2012·Medizinische Klinik, Intensivmedizin und Notfallmedizin·I BienerR Rossaint
Feb 19, 2013·Respiratory Physiology & Neurobiology·Matteo PecchiariEdgardo D'Angelo
Feb 22, 2011·Respiratory Physiology & Neurobiology·M CzaplikJ Bickenbach
Aug 23, 2011·Respiratory Physiology & Neurobiology·Johannes BickenbachMichael Fries
Jul 24, 2012·Respiratory Physiology & Neurobiology·Michael CzaplikJohannes Bickenbach

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