PMID: 11321436Apr 26, 2001Paper

In vivo microvascular actions of Artemisia vulgaris L. in a model of ischemia-reperfusion injury in the rat intestinal mesentery

Clinical Hemorheology and Microcirculation
X T Tigno, E Gumila

Abstract

Water extract fractions of leaves from Artemisia vulgaris L. (commonly known as mugwort) were tested for their effects on tissue damage brought about by ischemia-reperfusion injury in the rat mesentery. Male Sprague-Dawley rats, 200-300 grams in weight were divided into two groups, control and treatment (AV) group. All rats were anesthetized with ketamine HCl administered intramuscularly, tracheotomized and cannulated in one carotid artery and one jugular vein. After a midline abdominal incision, the mesenteric area was exteriorized and observed using videomicroscopy. After baseline observations of systemic blood pressure, heart rate, venular diameters and leukocyte adhesion along venules, the mesenteric artery and vein were occluded for 10 minutes. Prior to occlusion, A. vulgaris-treated animals were given a bolus injection of a 1% w/v solution of extracts, while the control group received saline. Monastral Blue dye was also administered before the occlusion at a dose of 30 mg/kg via the jugular vein in order to assess transendothelial leakage. Hemodynamic and cellular parameters were measured immediately after the release of occlusion and at 10 minute intervals thereafter. Results show that the extracts had no significant eff...Continue Reading

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.