In vivo mitotic spindle scaling can be modulated by changing the levels of a single protein: the microtubule polymerase XMAP215

Molecular Biology of the Cell
Ana Milunovic-JevticJ C Gatlin

Abstract

In many organisms, early embryonic development is characterized by a series of reductive cell divisions that result in rapid increases in cell number and concomitant decreases in cell size. Intracellular organelles, such as the nucleus and mitotic spindle, also become progressively smaller during this developmental window, but the molecular and mechanistic underpinnings of these scaling relationships are not fully understood. For the mitotic spindle, changes in cytoplasmic volume are sufficient to account for size scaling during early development in certain organisms. This observation is consistent with models that evoke a limiting component, whereby the smaller absolute number of spindle components in smaller cells limits spindle size. Here we investigate the role of a candidate factor for developmental spindle scaling, the microtubule polymerase XMAP215. Microinjection of additional XMAP215 protein into Xenopus laevis embryos was sufficient to induce the assembly of larger spindles during developmental stages 6.5, 7, and 8, whereas addition of a polymerase-incompetent XMAP215 mutant resulted in a downward shift in the in vivo spindle scaling curve. In sum, these results indicate that even small cells are able to produce large...Continue Reading

References

Feb 7, 2001·The EMBO Journal·A V PopovE Karsenti
Jan 15, 2008·Cell·Gary J BrouhardAnthony A Hyman
Aug 23, 2008·Current Biology : CB·Martin WührTimothy J Mitchison
Feb 9, 2010·Current Biology : CB·Garrett GreenanAnthony A Hyman
Oct 16, 2010·Cell·Daniel L Levy, Rebecca Heald
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Per O WidlundJonathon Howard
Aug 2, 2011·Current Biology : CB·Markus DeckerAnthony A Hyman
Dec 14, 2011·Cell·Rose LoughlinRebecca Heald
May 12, 2012·Current Biology : CB·Nathan W Goehring, Anthony A Hyman
Feb 22, 2013·ELife·Jeremy D Wilbur, Rebecca Heald
Aug 27, 2013·Nature Cell Biology·Simone B ReberFrank Jülicher
Nov 16, 2013·Science·Matthew C GoodRebecca Heald
Jun 27, 2014·Molecular Biology of the Cell·Jaime C FoxKevin C Slep
Aug 20, 2014·The Journal of Cell Biology·Lisa J Edens, Daniel L Levy
May 26, 2015·Current Biology : CB·Marina E CrowderRebecca Heald
Aug 9, 2015·Cold Spring Harbor Perspectives in Biology·Simone Reber, Nathan W Goehring
Sep 1, 2015·Nature Cell Biology·Sabine Petry, Ronald D Vale
Nov 12, 2015·Developmental Cell·Leonid PeshkinMarc W Kirschner
Feb 18, 2016·PloS One·Lucia NovakovaMartin Anger
Apr 14, 2017·Biophysical Journal·Jingchen Li, Hongyuan Jiang
May 10, 2017·Developmental Cell·Hirohisa Kyogoku, Tomoya S Kitajima
May 18, 2017·The Journal of Cell Biology·Amy E Byrnes, Kevin C Slep

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Citations

Feb 1, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Chase C WesleyDaniel L Levy
Jun 6, 2020·Essays in Biochemistry·Lara K Krüger, Phong T Tran
Aug 8, 2020·Biology of the Cell·Gabriel Guilloux, Romain Gibeaux
Dec 31, 2020·ELife·Jacob A HermanSue Biggins
Nov 21, 2020·Current Biology : CB·Elisa Maria RieckhoffJan Brugués
Jun 13, 2020·Current Biology : CB·Zachary M GeisterferJesse C Gatlin
Jun 10, 2020·Current Biology : CB·Daniel L Levy

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Software Mentioned

Olympus cellSens
ImageStudio
Igor Pro
Metamorph

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