In vivo mobility of a group I twintron in nuclear ribosomal DNA of the myxomycete Didymium iridis

Molecular Microbiology
Steinar JohansenF Haugli

Abstract

DiSSU1 is an optional group I twintron present in the nuclear extrachromosomal ribosomal DNA of the myxomycete Didymium iridis. DiSSU1 appears to be complex both in structure and function. At the RNA level it has a twin-ribozyme organization composed of two group I ribozymes with different functions, separated by an open reading frame. Here, we show that DiSSU1 is mobile when haploid intron-containing and intron-less amoebae are mated. The mobility process is fast, being completed in 5-10 nuclear cycles after mating in the developing zygote and plasmodia. Analyses of progeny from genetic crosses confirm intron mobility. DiSSU1 is the first example of a mobile group I twintron. The intron-encoded protein was expressed in Escherichia coli and found to be an endonuclease, I-DirI, that cleaves an intron-less ribosomal DNA allele at the intron-insertion site, and is probably involved in intron homing. The endonuclease I-DirI seems to be a rare example of a protein that is expressed from a ribozyme-processed RNA polymerase I transcript in vivo.

Citations

Nov 26, 2002·European Journal of Biochemistry·Anna VaderHenrik Nielsen
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Apr 9, 2005·Nucleic Acids Research·Asa B Birgisdottir, Steinar Johansen
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Aug 16, 2012·Genome Génome / Conseil National De Recherches Canada·Mohamed Hafez, Georg Hausner
May 4, 2006·BMC Evolutionary Biology·Odd-Gunnar WikmarkSteinar D Johansen
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Jun 7, 2013·Mobile DNA·Annica Hedberg, Steinar D Johansen
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Nov 17, 2009·Cellular and Molecular Life Sciences : CMLS·Maria J MarcaidaGuillermo Montoya
Oct 30, 2015·RNA Biology·Mohamed Hafez, Georg Hausner
Feb 16, 2007·The Journal of Eukaryotic Microbiology·Odd-Gunnar WikmarkSteinar D Johansen
Mar 12, 2011·Research in Microbiology·Lillian K Fritz-LaylinChandler Fulton
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Mar 17, 2021·Journal of Microbiological Methods·Wan WangYu Li

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