In vivo model of wound healing based on transplanted tissue-engineered skin

Tissue Engineering
David J GeerStelios T Andreadis

Abstract

Advances in understanding the complex process of wound healing and development of novel growth factor and gene therapies would benefit from models that mimic closely the physiology of human wounds. To this end, we developed a hybrid wound-healing model based on human tissue-engineered skin transplanted onto athymic mice. Grafted tissues were infiltrated with mouse mesenchymal cells as native and foreign dermal regions fused together. Immunohistochemical staining for human involucrin revealed that the transplanted epithelium maintained its human origin, whereas the dermis was infiltrated by numerous mouse fibroblasts and blood vessels. Grafted tissues were wounded with a 4-mm punch to create full-thickness excisional wounds. At 1 and 2 weeks, the tissues were excised and assessed for reepithelialization, differentiation, and neovascularization. Interestingly, the average rate of keratinocyte migration (120 microm/day) was similar to migration rates observed in human subjects and significantly lower than migration in mouse epidermis. Immunohistochemical staining for keratin 10, laminin, and involucrin revealed a normal pattern of differentiation in the neoepidermis. Neovascularization was significantly elevated in the granulation...Continue Reading

References

Nov 1, 1992·The Journal of Dermatology·R TsuboiH Ogawa
Jun 1, 1992·The Journal of Clinical Investigation·M D HertleF M Watt
Sep 1, 1987·The Journal of Investigative Dermatology·J N Mansbridge, A M Knapp
Jul 1, 1985·The British Journal of Dermatology·M DemarchezM Prunieras
Sep 1, 1993·The Journal of Clinical Investigation·H LarjavaJ Heino
Oct 1, 1993·The Journal of Investigative Dermatology·A CavaniA Giannetti
Sep 1, 1996·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·T ShimonoI Yada
Jan 1, 1997·Cell Motility and the Cytoskeleton·J M ZahmE Puchelle
Jul 18, 1997·Biochemical and Biophysical Research Communications·P A Coulombe
Sep 1, 1997·The Journal of Investigative Dermatology·M PonecA M Mommaas
Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T KainulainenA Oikarinen
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T M KolodkaL B Taichman
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A F BlackF A Auger
Oct 20, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·B C NwomehD R Yager
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Sep 7, 1999·In Vitro Cellular & Developmental Biology. Animal·M MichelL Germain
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·M G TonnesenR A Clark
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S T AndreadisJ R Morgan
May 15, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·T P SullivanP Mertz
Nov 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A F LaplanteV Moulin
Nov 27, 2001·Science·E CukiermanK M Yamada
Mar 8, 2002·The Journal of Burn Care & Rehabilitation·A GragnaniL M Ferreira
Apr 23, 2002·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Yuji YamaguchiSatoshi Itami
May 2, 2002·The Journal of Investigative Dermatology·Stacy MazzalupoPierre A Coulombe
Aug 8, 2002·The Journal of Investigative Dermatology·Laurent TurchiGilles Ponzio
Aug 23, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nicola J BrownMalcolm W R Reed
Nov 5, 2002·Journal of Cell Science·Mary Ann SteppMerton Bernfield
Dec 3, 2002·Tissue Engineering·David J GeerStelios T Andreadis
Jan 8, 2004·The Journal of Investigative Dermatology·David J Geer, Stelios T Andreadis
Apr 5, 1994·Biotechnology and Bioengineering·L M WilkinsN L Parenteau

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Citations

Mar 1, 1977·American Journal of Human Genetics·M Melnick, D Bixler
Sep 26, 2006·Tissue Engineering·Ken KobayashiKoichi Omori
Dec 15, 2010·Journal of Biomedicine & Biotechnology·Victor W WongGeoffrey C Gurtner
Oct 19, 2011·Cells, Tissues, Organs·Haofan PengDaniel D Swartz
Mar 17, 2010·Archives of Dermatological Research·Alexandra L Varol, Chris D Anderson
Oct 20, 2006·Expert Opinion on Biological Therapy·Stelios T Andreadis
Jul 15, 2011·The Journal of Investigative Dermatology·Satomi Nishikawa-TorikaiShin-Ichi Nishikawa
Apr 29, 2006·The Journal of Investigative Dermatology·Piyush Koria, Stelios T Andreadis
Mar 27, 2009·Experimental Dermatology·Christian TemplinJörg Faulhaber
Nov 17, 2007·Journal of Anatomy·Heiko SorgBrigitte Vollmar
Feb 27, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Nicholas S GreavesArdeshir Bayat
May 24, 2006·Trends in Biotechnology·Stelios T Andreadis, David J Geer
May 7, 2013·Annual Review of Biomedical Engineering·François A AugerDan Lacroix
Oct 19, 2007·Toxicologic Pathology·Liora Braiman-WiksmanTamar Tennenbaum
Aug 16, 2015·The Annals of Otology, Rhinology, and Laryngology·Yuta NakaegawaKoichi Omori
Aug 23, 2011·The International Journal of Lower Extremity Wounds·Su LuoVincent Falanga
Nov 29, 2016·Experimental Dermatology·Jie WenXunwei Wu
Oct 5, 2007·Cell Transplantation·Neeltje A CoolenMagda M W Ulrich
May 24, 2007·Tissue Engineering·Kevin M SchusterCharles W Hewitt
Oct 16, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Daniel D SwartzStelios T Andreadis
Feb 28, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ryosuke NakamuraKoichi Omori
Oct 24, 2018·Plastic and Reconstructive Surgery. Global Open·Peter ApelgrenLars Kölby
Apr 1, 2020·International Journal of Experimental Pathology·Daniela S Masson-MeyersMarco Andrey C Frade
Jul 1, 2007·Cell Transplantation·Neeltje A CoolenMagda M W Ulrich
Mar 16, 2013·Experimental Dermatology·Lucía Martínez-SantamaríaMarcela Del Río
Apr 1, 2020·International Wound Journal·Dominic HennGeoffrey C Gurtner
Feb 21, 2021·Advances in Wound Care·Meilang XueChristopher Jackson
Aug 3, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bharat Kumar Reddy SanapalliVeera Venkata Satyanarayana Reddy Karri

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