In vivo mutagenicity of arsenite in the livers of gpt delta transgenic mice

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Shota TakumiKeiko Nohara

Abstract

While arsenic has been classified as a Group 1 human carcinogen by the International Agency for Research on Cancer (IARC), its mutagenicity has not been fully characterized in experimental animals. The aim of this study was to assess the in vivo mutagenicity of arsenite in C57BL/6J gpt delta mice. Male gpt delta mice were given drinking water containing sodium arsenite for 3 weeks, and the hepatic genome was assayed for mutations 2 weeks later. The gpt mutation assays showed a significant increase in mutation frequency in the liver following arsenite exposure. Sequence analysis revealed that 67% of mutations detected are G:C to A:T transitions and 5% are G:C to T:A transversions in the control group, and arsenite exposure resulted in a markedly higher rate of G:C to T:A transversions (46% of mutations detected). G:C to T:A transversions have been reported to be induced following formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a representative product that results from oxidative DNA damage. We also detected a significant increase in 8-OHdG in the livers of the mice exposed to arsenite. These results demonstrate that arsenite has mutagenicity in vivo and suggest that arsenite induces G:C to T:A transversions through oxidative-...Continue Reading

References

Oct 9, 1980·Nature·B K Duncan, J H Miller
Jan 1, 1996·Environmental and Molecular Mutagenesis·T NohmiT Sofuni
Dec 13, 2000·Mutation Research·T NohmiK Masumura
Nov 24, 2001·Mutation Research·Yasuhiro NodaShoji Okada
Nov 12, 2002·Environmental Health Perspectives·Toby G RossmanMaarten C Bosland
Dec 4, 2003·Mutation Research·Toby G Rossman
Jan 20, 2005·Environmental and Molecular Mutagenesis·Akiko H HashimotoYasunobu Aoki
Mar 18, 2008·Free Radical Biology & Medicine·Shota TakumiToru Takeuchi
Dec 6, 2008·The Journal of Biological Chemistry·Wei DingKe Jian Liu
Jan 24, 2009·Biochemistry·Diana V MaltsevaElizaveta S Gromova
Feb 16, 2011·Journal of Applied Toxicology : JAT·K JomovaM Valko
May 11, 2011·Free Radical Biology & Medicine·Swaran J S Flora
Jul 14, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael F HughesDavid J Thomas
Oct 25, 2011·Trends in Genetics : TIG·Sébastien A Smallwood, Gavin Kelsey
Jan 15, 2013·Environment International·Pritha BhattacharjeeAshok K Giri

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Citations

Mar 20, 2015·Archives of Toxicology·Kazuyuki Okamura, Keiko Nohara
Aug 9, 2016·Biological Trace Element Research·Mengchuan XuMingxia Jing
Jan 1, 2015·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Yasunobu AokiTakehiko Nohmi
Mar 8, 2017·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Keiko NoharaShota Takumi
Apr 17, 2021·Scientific Reports·Lydia Enith Nava-RiveraJavier Morán-Martínez

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