In vivo mutation analysis using the ΦX174 transgenic mouse and comparisons with other transgenes and endogenous genes

Mutation Research
Carrie R ValentineRobert H Heflich

Abstract

The ΦX174 transgenic mouse was first developed as an in vivo Ames test, detecting base pair substitution (bps) at a single bp in a reversion assay. A forward mutational assay was also developed, which is a gain of function assay that also detects bps exclusively. Later work with both assays focused on establishing that a mutation was fixed in vivo using single-burst analysis: determining the number of mutant progeny virus from an electroporated cell by dividing the culture into aliquots before scoring mutants. We review results obtained from single-burst analysis, including testing the hypothesis that high mutant frequencies (MFs) of G:C to A:T mutation recovered by transgenic targets include significant numbers of unrepaired G:T mismatches. Comparison between the ΦX174 and lacI transgenes in mouse spleen indicates that the spontaneous bps mutation frequency per nucleotide (mf(n)) is not significantly lower for ΦX174 than for lacI; the response to ENU is also comparable. For the lacI transgene, the spontaneous bps mf(n) is highly age-dependent up to 12 weeks of age and the linear trend extrapolates at conception to a frequency close to the human bps mf(n) per generation of 1.7 × 10(-8). Unexpectedly, we found that the lacI soma...Continue Reading

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L A Weymouth, L A Loeb
Oct 25, 1978·Journal of Molecular Biology·F SangerM Smith
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J W Drake
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A GossenJ Vijg
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T P BrentP M Potter
Jul 1, 1966·Journal of Molecular Biology·C A Hutchison, R L Sinsheimer
Jan 1, 1980·Annual Review of Genetics·I Herskowitz, D Hagen
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·G R DouglasL M Soper
Jan 1, 1995·Environmental and Molecular Mutagenesis·T R SkopekD R Marino
Jan 1, 1995·Environmental and Molecular Mutagenesis·W W PiegorschK R Tindall
Apr 1, 1995·Mutation Research·T G RossmanA Nádas
May 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A T LeeR Bucala
Feb 1, 1996·Mutation Research·T R SkopekT Pippert
Jul 5, 1996·Mutation Research·M Khaidakov, B W Glickman
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J L JakubczakS Garges
Jan 1, 1996·Environmental and Molecular Mutagenesis·T NohmiT Sofuni
Sep 5, 1997·Science·F R BlattnerY Shao
May 6, 1998·Mutagenesis·J G de BoerB W Glickman
Dec 23, 1998·Mutagenesis·Y R Paashuis-Lew, J A Heddle
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BachlC Steinberg
Aug 18, 2000·Mutation Research·W Cruz-MunozJ A Heddle
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·J H Bielas, J A Heddle
Dec 13, 2000·Mutation Research·T NohmiK Masumura
Mar 27, 2001·Nature Reviews. Genetics·J F Crow
Jun 26, 2001·Environmental and Molecular Mutagenesis·H V Malling, R R Delongchamp
Mar 29, 2002·Journal of Cellular Physiology·Thomas M MartiOliver Fleck
May 29, 2002·Molecular Pathology : MP·R J Johnson, P Hillmen
Aug 2, 2002·Molecular Cell·Youri I PavlovThomas A Kunkel
Oct 16, 2002·Mutation Research·Hironobu Ikehata, Tetsuya Ono
Dec 19, 2002·Environmental and Molecular Mutagenesis·Lois B BarnettSusan E Lewis

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Citations

Jun 29, 2012·Journal of Virology·Pilar Domingo-CalapRafael Sanjuán
Oct 4, 2016·Environmental and Molecular Mutagenesis·George R HoffmannErrol Zeiger

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