In vivo nano-imaging of membrane dynamics in metastatic tumor cells using quantum dots.

The Journal of Biological Chemistry
Kohsuke GondaHideo Higuchi

Abstract

Changes in membrane morphology and membrane protein dynamics based on its fluidity are critical for cancer metastasis. However, this subject has remained unclear, because the spatial precision of previous in vivo imaging has been limited to the micrometer level and single molecule imaging is impossible. Here, we have imaged the membrane dynamics of tumor cells in mice with a spatial precision of 7-9 nm under a confocal microscope. A metastasis-promoting factor on the cell membrane, protease-activated receptor 1 (PAR1), was labeled with quantum dots conjugated with an anti-PAR1 antibody. Movements of cancer cells and PAR1 during metastasis were clearly observed in vivo. Images used to assess PAR1 dynamics were taken of representative cells for four stages of metastasis; i.e. cancer cells far from blood vessels in tumor, near the vessel, in the bloodstream, and adherent to the inner vascular surface in the normal tissues near tumor were photographed. The diffusion constant of PAR1 in static cells far from tumor blood vessels was smaller than in moving cells near the vessels and in the bloodstream. The diffusion constant of cells adhering to the inner vascular surface in the normal tissues was also very small. Cells formed membran...Continue Reading

References

Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Jan 6, 2004·Nature Biotechnology·Diane S LidkeThomas M Jovin
Jan 23, 2004·Nature Reviews. Cancer·John Condeelis, Jeffrey E Segall
Feb 18, 2004·Molecular and Cellular Biology·Michelle A BoodenJoAnn Trejo
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Wenjun Guo, Filippo G Giancotti
Feb 3, 2005·The Journal of Cell Biology·Hideki YamaguchiJohn Condeelis
Aug 16, 2005·Current Opinion in Cell Biology·Hideki YamaguchiJohn Condeelis
Oct 1, 2005·Nature Reviews. Cancer·Robert M Hoffman
Dec 2, 2005·Nature·Frederick R Maxfield, Ira Tabas
Mar 25, 2006·Nature Reviews. Cancer·Pepper Schedin
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shiori TobaHideo Higuchi
Oct 24, 2006·Biochemical and Biophysical Research Communications·Songhua Li-ShishidoNoriaki Ohuchi
Jan 26, 2007·Nature Reviews. Cancer·Ignacio MeleroLieping Chen
Mar 9, 2007·Journal of Cell Science·Puneeta AroraJoAnn Trejo
Mar 21, 2007·Biophysical Journal·Tomonobu M Watanabe, Hideo Higuchi
Sep 25, 2007·Nature Reviews. Cancer·Erik Sahai
Mar 26, 2008·The Journal of Cell Biology·Corina SarmientoJohn Condeelis
May 10, 2008·Nature Reviews. Molecular Cell Biology·Pieta K Mattila, Pekka Lappalainen
Jul 29, 2008·Biochemical and Biophysical Research Communications·Q S LiC T Lim
Sep 2, 2008·Nature Reviews. Drug Discovery·Mark E DavisDong M Shin
Oct 14, 2008·Experimental Cell Research·Jungwoo YooHideo Higuchi

❮ Previous
Next ❯

Citations

May 10, 2012·Journal of Biomedicine & Biotechnology·Fuyu Kobirumaki-ShimozawaNorio Fukuda
Mar 8, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Margarida M Barroso
Sep 21, 2011·International Journal of Nanomedicine·Roman GeneralovPetras Juzenas
Mar 15, 2011·Immunotherapy·Francois Fay, Christopher J Scott
Mar 19, 2014·The Journal of General Physiology·Seine A ShintaniNorio Fukuda
Feb 19, 2013·The Journal of Membrane Biology·Vladimir AjdžanovićIvan Spasojević
Dec 7, 2010·Histochemistry and Cell Biology·Minenori Ishido, Norikatsu Kasuga
Jul 12, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Balint CsobozLaszlo Vigh
Dec 29, 2015·Biotechnology Journal·Abhishek MukherjeeJoon Myong Song
Mar 7, 2012·Medical Hypotheses·Carla C C R de Carvalho, Maria José Caramujo
Feb 7, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·Hironori UenoTakami Yamaguchi
Nov 23, 2011·Cancer Letters·Sukhwinder KaurSurinder K Batra
Feb 8, 2011·Advanced Drug Delivery Reviews·Omid VeisehMiqin Zhang
Jul 27, 2010·Biophysical Journal·Saame Raza Shaikh
Aug 13, 2013·Biophysical Journal·Tomonobu M WatanabeToshio Yanagida
Jan 27, 2015·Cell and Tissue Research·Tania Q VuDiane S Lidke
May 21, 2016·Frontiers in Cell and Developmental Biology·Arnauld Sergé
Jun 9, 2016·Advanced Drug Delivery Reviews·Miles A Miller, Ralph Weissleder
Oct 22, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Hamid Reza SamadikhahSaman Hosseinkhani
Mar 18, 2015·Chemical Society Reviews·K David Wegner, Niko Hildebrandt
Jun 10, 2015·Chemical Society Reviews·Qianjun HeXiaoyuan Chen
Oct 11, 2016·Biomarkers in Cancer·Martin C BoonstraCornelis F M Sier
Jun 20, 2018·Nanoscale·Elisabetta AvitabileLucia Gemma Delogu
Apr 15, 2010·Nanotechnology·Makoto HikageNoriaki Ohuchi
Jun 24, 2017·Nature Reviews. Cancer·Miles A Miller, Ralph Weissleder
Jan 22, 2019·Frontiers in Bioengineering and Biotechnology·Hideaki FujitaMadoka Suzuki
May 10, 2018·Nature Communications·Mohammad U ZahidAndrew M Smith
Apr 14, 2011·ACS Medicinal Chemistry Letters·Yuji KomizuRyuichi Ueoka

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.