In Vivo Near-Infrared Fluorescence Imaging of Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes

Scientific Reports
Marie-Jeanne BertrandJean-Claude Tardif

Abstract

This study aimed to evaluate the feasibility and accuracy of a technique for atherosclerosis imaging using local delivery of relatively small quantities (0.04-0.4 mg/kg) of labeled-specific imaging tracers targeting ICAM-1 and unpolymerized type I collagen or negative controls in 13 rabbits with atheroma induced by balloon injury in the abdominal aorta and a 12-week high-cholesterol diet. Immediately after local infusion, in vivo intravascular ultrasonography (IVUS)-NIRF imaging was performed at different time-points over a 40-minute period. The in vivo peak NIRF signal was significantly higher in the molecular tracer-injected rabbits than in the control-injected animals (P < 0.05). Ex vivo peak NIRF signal was significantly higher in the ICAM-1 probe-injected rabbits than in controls (P = 0.04), but not in the collagen probe-injected group (P = 0.29). NIRF signal discrimination following dual-probe delivery was also shown to be feasible in a single animal and thus offers the possibility of combining several distinct biological imaging agents in future studies. This innovative imaging strategy using in vivo local delivery of low concentrations of labeled molecular tracers followed by IVUS-NIRF catheter-based imaging holds poten...Continue Reading

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Citations

Jun 15, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Anastasia KougioumtziAndreas G Tzakos
Feb 5, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Miao LiWeibo Cai

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Methods Mentioned

BETA
fluorescence reflectance imaging
fluorescence imaging
confocal microscopy
imaging technique
size-exclusion
surface plasmon resonance
ELISA
fluorescence microscopy

Software Mentioned

GraphPad
Prism
Syngo
Lumina II Living Image
Matlab

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