In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system

Nature Communications
Guillaume Petit-PierrePhilippe Renaud

Abstract

Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.

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Citations

May 29, 2021·Biosensors & Bioelectronics·Yuqian ZhangAli K Yetisen
May 13, 2021·Annual Review of Analytical Chemistry·Nan ShiChristopher J Easley
Dec 24, 2021·ACS Measurement Science Au·Sara E BellJonathan V Sweedler

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