In vivo non-invasive monitoring of collagen remodelling by two-photon microscopy after micro-ablative fractional laser resurfacing

Journal of Biophotonics
Riccardo CicchiFrancesco Saverio Pavone

Abstract

Non-linear optical microscopy is becoming popular as a non-invasive in vivo imaging modality in dermatology. In this study, combined TPF and SHG microscopy were used to monitor collagen remodelling in vivo after micro-ablative fractional laser resurfacing. Papillary dermis of living subjects, covering a wide age range, was imaged immediately before and forty days after treatment. A qualitative visual examination of acquired images demonstrated an age-dependent remodelling effect on collagen. Additional quantitative analysis of new collagen production was performed by means of two image analysis methods. A higher increase in SHG to TPF ratio, corresponding to a stronger treatment effectiveness, was found in older subjects, whereas the effect was found to be negligible in young, and minimal in middle age subjects. Analysis of collagen images also showed a dependence of the treatment effectiveness with age but with controversial results. While the diagnostic potential of in vivo multiphoton microscopy has already been demonstrated for skin cancer and other skin diseases, here we first successfully explore its potential use for a non-invasive follow-up of a laser-based treatment.

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Citations

Sep 25, 2014·BioMed Research International·Riccardo CicchiFrancesco Saverio Pavone
Sep 22, 2015·Lasers in Surgery and Medicine·Urška B OgrincHelena Lenasi
Aug 12, 2016·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·J H YimM K Shin
Oct 12, 2018·Journal of Biophotonics·Jyh-Hong LeeBor-Luen Chiang
Dec 19, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ling YutingGhulam Nabi
Jul 3, 2015·Biomedical Optics Express·Ben SherlockChris Dunsby
Nov 10, 2018·Dermatologic Therapy·Paolo AmoriTorello Lotti
Jun 26, 2021·Biomedical Optics Express·Chung-Hwan ChenChi-Hsiang Lien
Aug 8, 2021·Journal of Clinical Medicine·Paulina Szczepanik-KułakDorota Krasowska

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