PMID: 2105661Jan 11, 1990Paper

In vivo osmoregulation of aldose reductase mRNA, protein, and sorbitol in renal medulla

The American Journal of Physiology
B D CowleyM B Burg

Abstract

Sorbitol accumulates in renal medullary cells by synthesis from glucose in a reaction catalyzed by aldose reductase. Medullary sodium and urea are high and vary with urinary concentration. Sorbitol varies similarly, consistent with its role as a compatible intracellular organic osmolyte. We measured renal medullary sodium, urea, sorbitol, aldose reductase (protein and activity), and aldose reductase mRNA in rats treated to change medullary sodium and urea. In untreated Brattleboro rats all measurements were low and increased after 7 days of treatment with arginine vasopressin. In contrast, when normal rats were water deprived for 3 days, urea increased out of proportion to sodium, and sorbitol, aldose reductase, and aldose reductase mRNA were unchanged. After 2 h of diuresis, normal rats had lower medullary sodium and urea and reduced mRNA and sorbitol; however aldose reductase did not change. These data are consistent with previous results from cultured cells in which altered extracellular sodium, but not urea, leads to rapid changes in aldose reductase mRNA and slow changes (days) in aldose reductase. In addition, acute decreases in extracellular sodium increase leakage of sorbitol from cells. We also confirm previous results...Continue Reading

Citations

May 23, 2006·Physiology·Wolfgang Neuhofer, Franz-X Beck
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Ju-Young JungJin Kim
Mar 11, 2000·American Journal of Physiology. Renal Physiology·D Sheikh-HamadY Yang
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Ming-Zhi ZhangJames A McKanna

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