In Vivo Periodontium Formation Around Titanium Implants Using Periodontal Ligament Cell Sheet

Tissue Engineering. Part a
Kaoru WashioIsao Ishikawa

Abstract

Osseointegrated implants have been recognized as being very reliable and having long-term predictability. However, host defense mechanisms against infection have been known to be impaired around a dental implant because of the lack of a periodontal ligament (PDL). The purpose of our experimental design was to produce cementum and PDL on the implant surface adopting cell sheet technology. To this aim we used PDL-derived cells, which contain multipotential stem cells, as the cell source and we cultured them on an implant material constituted of commercially pure titanium treated with acid etching, blasting, and a calcium phosphate (CaP) coating to improve cell attachment. Implants with adhered human PDL cell sheets were transplanted into bone defects in athymic rat femurs as a xenogeneic model. Implants with adhered canine PDL-derived cell sheets were transplanted into canine mandibular bone as an autologous model. We confirmed that PDL-derived cells cultured with osteoinductive medium had the ability to induce cementum formation. The attachment of PDL cells onto the titanium surface with three surface treatments was accelerated, compared with that onto the smooth titanium surface, at 40 min after starting incubation. Results in ...Continue Reading

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Citations

Jan 11, 2019·Cells·Smaranda Dana BuduruIoana Berindan-Neagoe
Jan 17, 2019·Tissue Engineering. Part a·Zhifang WuSonglin Wang
Jul 19, 2020·Journal of Clinical Medicine·Dobrila NesicIrena Sailer
Sep 26, 2020·Frontiers in Cell and Developmental Biology·Anqi ZhouLin Xiang
Oct 20, 2020·Biomaterials Science·Yuting FengGuoli Yang
Sep 25, 2019·The Journal of Prosthetic Dentistry·Ahmad Mahmood TahirMatteo Zoppi
Nov 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiaqi BaoLili Chen
Apr 23, 2021·Tissue Engineering. Part B, Reviews·Francesca MangioneSibylle Vital
Jul 13, 2021·World Journal of Stem Cells·Aline QueirozMarinella Holzhausen
Sep 4, 2021·Tissue Engineering. Part a·Lisa BerntsenDaniel J Hayes

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Methods Mentioned

BETA
PCR
transmission electron microscopy
light microscopy

Software Mentioned

Elements D
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