In vivo phosphorylation of partner switching regulators correlates with stress transmission in the environmental signaling pathway of Bacillus subtilis

Journal of Bacteriology
Tae-Jong KimChester W Price

Abstract

Exposure of bacteria to diverse growth-limiting stresses induces the synthesis of a common set of proteins which provide broad protection against future, potentially lethal stresses. Among Bacillus subtilis and its relatives, this general stress response is controlled by the sigmaB transcription factor. Signals of environmental and energy stress activate sigmaB through a multicomponent network that functions via a partner switching mechanism, in which protein-protein interactions are governed by serine and threonine phosphorylation. Here, we tested a central prediction of the current model for the environmental signaling branch of this network. We used isoelectric focusing and immunoblotting experiments to determine the in vivo phosphorylation states of the RsbRA and RsbS regulators, which act in concert to negatively control the RsbU environmental signaling phosphatase. As predicted by the model, the ratio of the phosphorylated to unphosphorylated forms of both RsbRA and RsbS increased in response to salt or ethanol stress. However, these two regulators differed substantially with regard to the extent of their phosphorylation under both steady-state and stress conditions, with RsbRA always the more highly modified. Mutant anal...Continue Reading

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Citations

Aug 9, 2011·Nature Reviews. Microbiology·Julien Herrou, Sean Crosson
Jul 8, 2009·The Journal of Biological Chemistry·Marcela Avila-PérezKlaas J Hellingwerf
Aug 25, 2009·The Journal of Biological Chemistry·Andrew E GreensteinTom Alber
Aug 23, 2006·Journal of Bacteriology·Tatiana A GaidenkoChester W Price
Jun 26, 2007·Journal of Bacteriology·Adam ReevesWilliam Haldenwang
Feb 10, 2009·Journal of Bacteriology·Jan Pané-FarréSusanne Engelmann
Nov 18, 2005·Journal of Bacteriology·Maria Magdalena SennMarkus Bischoff
Dec 3, 2005·Journal of Bacteriology·Shuyu ZhangWilliam Haldenwang
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Jan 17, 2013·BMC Systems Biology·Ulf W LiebalOlaf Wolkenhauer
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May 15, 2007·Journal of Molecular Biology·Oleg A IgoshinMichael A Savageau
Aug 18, 2004·Journal of Molecular Biology·Tae-Jong KimChester W Price
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Related Concepts

SigB protein, Bacteria
RsbU protein, Bacillus subtilis
RsbR protein, Bacillus subtilis
RsbT protein, Bacillus subtilis
Adaptation, Physiological
Ethanol
Natto Bacteria
Bacterial Proteins
Growth Inhibitors
Isoelectric Focusing

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