In Vivo Photodynamic Therapy With a Lipophilic Zinc(II) Phthalocyanine Inhibits Colorectal Cancer and Induces a Th1/CD8 Antitumor Immune Response.

Lasers in Surgery and Medicine
Nicolás ChiaranteLeonor P Roguin

Abstract

Photodynamic therapy (PDT) is an antitumor procedure clinically approved for the treatment of different cancer types. Despite strong efforts and promising results in this field, PDT has not yet been approved by any regulatory authority for the treatment of colorectal cancer, one of the most prevalent gastrointestinal tumors. In the search of novel therapeutic strategies, we examined the in vivo effect of PDT with a lipophilic phthalocyanine (Pc9) encapsulated into polymeric poloxamine micelles (T1107) in a murine colon carcinoma model. In vivo assays were performed with BALB/c mice challenged with CT26 cells. Pc9 tumor uptake was evaluated with an in vivo imaging system. Immunofluorescence, western blot, and flow cytometry assays were carried out to characterize the activation of apoptosis and an antitumor immune response. Pc9-T1107 effectively delayed tumor growth and prolonged mice survival, without generating systemic or tissue-specific toxicity. The induction of an apoptotic response was characterized by a decrease in the expression levels of Bcl-XL , Bcl-2, procaspase 3, full length Bid, a significant increment in the amount of active caspase-3 and the detection of PARP-1 cleavage. Infiltration of CD8+ CD107a+ T cells and ...Continue Reading

References

Jun 1, 1987·Photochemistry and Photobiology·T J Dougherty
Mar 19, 2002·Journal of Photochemistry and Photobiology. B, Biology·Yvette Niamien KonanEric Allémann
Nov 2, 2002·Blood·Muneo NumasakiMichael T Lotze
Jul 23, 2004·Journal of Medicinal Chemistry·Michael R DettyStephen J Wagner
Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·A Rodríguez-HernándezJ A Sánchez-Alcázar
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Paul O Hassa, Michael O Hottiger
Sep 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Minfang ZhangMasako Yudasaka
Jun 1, 2010·Photodiagnosis and Photodynamic Therapy·Ron R Allison, Claudio H Sibata
Jul 6, 2010·European Journal of Medicinal Chemistry·Julieta MarinoJosefina Awruch
Aug 10, 2010·Chemical Society Reviews·Manivannan EthirajanRavindra K Pandey
May 28, 2011·CA: a Cancer Journal for Clinicians·Patrizia AgostinisJakub Golab
Nov 30, 2011·Photodiagnosis and Photodynamic Therapy·Renata SanovicBarbara Krammer
Feb 23, 2012·Carcinogenesis·Yil Sik HyunHo-Youn Kim
Aug 29, 2012·Photochemistry and Photobiology·María C García ViorJosefina Awruch
Mar 19, 2014·Photodiagnosis and Photodynamic Therapy·Seong Taek MunWoong Shick Ahn
May 14, 2014·Advances in Experimental Medicine and Biology·Naveena B Janakiram, Chinthalapally V Rao
Jun 6, 2014·Journal of Immunology Research·Glauben LandskronMarcela A Hermoso
Oct 17, 2014·Clinical and Molecular Hepatology·Ankush SharmaChandana Lall
Jan 7, 2015·The Journal of Clinical Investigation·Christoph ReissfelderPhilipp Beckhove
May 2, 2015·Photodiagnosis and Photodynamic Therapy·Aleksandra Kawczyk-KrupkaAleksander Sieroń
Aug 5, 2015·Photodiagnosis and Photodynamic Therapy·Aleksandra Kawczyk-KrupkaAleksander Sieroń
Sep 30, 2015·Bioscience Reports·Zenildo Santos SilvaMichael R Hamblin
Dec 25, 2015·PLoS Neglected Tropical Diseases·Elisangela Oliveira FreitasClarisa B Palatnik-de-Sousa
Feb 10, 2016·Frontiers in Physiology·Divya SinghGhanshyam Upadhyay
Sep 2, 2016·Methods in Molecular Biology·Yoon Kow YoungKoren K Mann

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Dec 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marina PorchiaCarlo Santini
Dec 11, 2021·Analytical Chemistry·Dong Joon LeeHwan Myung Kim

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