In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors

Proceedings of the National Academy of Sciences of the United States of America
Tommaso TorcellanTatyana Chtanova

Abstract

Immune therapy is rapidly gaining prominence in the clinic as a major weapon against cancer. Whereas much attention has been focused on the infiltration of tumors by immune cells, the subsequent fate of these infiltrates remains largely unexplored. We therefore established a photoconversion-based model that allowed us to label tumor-infiltrating immune cells and follow their migration. Using this system, we identified a population of tumor-experienced cells that emigrate from primary tumors to draining lymph nodes via afferent lymphatic vessels. Although the majority of tumor-infiltrating cells were myeloid, T cells made up the largest population of tumor-egressing leukocytes. Strikingly, the subset composition of tumor-egressing T cells was greatly skewed compared with those that had infiltrated the tumor and those resident in the draining lymph node. Some T-cell subsets such as CD8+ T cells emigrated more readily; others including CD4-CD8- T cells were preferentially retained, suggesting that specific mechanisms guide immune cell egress from tumors. Furthermore, tumor-egressing T cells were more activated and displayed enhanced effector function in comparison with their lymph node counterparts. Finally, we demonstrated that t...Continue Reading

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Citations

Feb 13, 2018·Chemistry : a European Journal·Manuel Alexander Mohr, Periklis Pantazis
Nov 30, 2019·Nature Reviews. Cancer·Gautier FollainJacky G Goetz
Sep 1, 2017·The FEBS Journal·Peter J Murray
Jul 10, 2018·Biomedical Optics Express·Sheldon J J KwokSeok-Hyun Yun
Mar 13, 2018·Frontiers in Oncology·Dennis JonesTimothy P Padera
Jun 14, 2019·Frontiers in Immunology·Henry R Hampton, Tatyana Chtanova
Mar 23, 2019·Frontiers in Immunology·Andrew O Yam, Tatyana Chtanova
May 29, 2020·Journal for Immunotherapy of Cancer·Thomas LaSalleUmar Mahmood
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Jan 17, 2021·International Journal of Molecular Sciences·Vincent Q SierMunitta Muthana
Jan 6, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria M Steele, Amanda W Lund
Apr 2, 2018·Current Opinion in Immunology·Qiaoli MaMichael Detmar
Mar 23, 2021·Immunotherapy Advances·Gessa SugiyartoEdward James
Jan 1, 2020·Seminars in Cancer Biology·Lothar C Dieterich, Andreas Bikfalvi
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Jul 23, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryoyo IkebuchiMichio Tomura
Jun 9, 2021·Cancer Research·Nikola CousinLothar C Dieterich
Oct 16, 2021·Clinical & Experimental Metastasis·Stanley P LeongAmanda W Lund

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