In vivo platelet deposition on polytetrafluoroethylene coated with fibrin glue containing fibroblast growth factor 1 and heparin in a canine model

American Journal of Surgery
C GosselinH P Greisler

Abstract

We previously reported that the coating of expanded polytetrafluoroethylene (ePTFE) with fibrin glue containing fibroblast growth factor 1 (FGF-1) and heparin accelerates endothelial coverage of grafts implanted into animals. We report here the effect of this surface modification on early platelet deposition. Nine dogs received 7-cm ePTFE grafts, 60-microns internodal distance, 4-mm internal diameter, as bilateral aortoiliac implants, one coated (luminal cross section and abluminal surface) with fibrin glue (fibrinogen 32.1 mg/mliters, thrombin 0.32 U/mliters) containing FGF-1 (11 ng/mliters and heparin (250 U/mliters), the other uncoated. After 5, 30, or 120 minutes of circulation with blood containing autologous platelets radiolabelled with indium 111, gamma emissions were quantitated on explants and correlated to surface areas measured by computerized planimetry. Both global and segmental comparisons showed significantly (P < 0.05, Student's t-test) less platelet deposition on coated than on uncoated grafts after 120 minutes of circulation, but no difference at 5 and 30 minutes. In this model, ePTFE coating with fibrin glue containing FGF-1 and heparin shows no adverse effect on early platelet deposition.

References

Sep 1, 1991·Annals of Vascular Surgery·M B HerringK J Etchberger
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Aug 1, 1990·Plastic and Reconstructive Surgery·P C Johnson
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Citations

Aug 16, 2000·Advanced Drug Delivery Reviews·S A Parikh, E R Edelman
Mar 27, 2001·Journal of Biomaterials Science. Polymer Edition·A K Tassiopoulos, H P Greisler
Dec 20, 2008·Journal of Biomedical Materials Research. Part a·Mary B Chan-ParkHoward P Greisler
May 22, 2007·Advanced Drug Delivery Reviews·Ge Zhang, Laura J Suggs
Oct 31, 2006·American Journal of Surgery·Luke BrewsterHoward P Greisler
Mar 20, 2004·Expert Review of Cardiovascular Therapy·Luis Leon, Howard P Greisler
Mar 25, 2006·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Tomomi HasegawaYutaka Okita
Mar 7, 2006·Expert Review of Medical Devices·Gemma ConnAlexander M Seifalian

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