In vivo protection against Tityus serrulatus scorpion venom by antibodies raised against a discontinuous synthetic epitope

Vaccine
Clara Guerra DuarteCarlos Chávez-Olórtegui

Abstract

Scorpion stings cause human fatalities in numerous countries. Serotherapy is the only specific means to try to circumvent the noxious effects of venom toxins. TsNTxP is a natural anatoxin from the venom of the scorpion Tityus serrulatus that may be useful to raise therapeutic anti-venom sera. Linear epitopes recognized by anti-TsNTxP antibodies have previously been mapped. Here, we attempted to identify discontinuous epitopes in TsNTxP since neutralizing epitopes are often associated with such complex entities. One hundred and fifty-three octadecapeptides with the general formula (P1)-(Gly-Gly)-(P2) were synthesized by the Spot method on cellulose membranes. P1 and P2 were octapeptides from the TsNTxP N-terminal and C-terminal sections, respectively. Each sequence of eight amino acids was frameshifted in turn by three residues, in order to cover TsNTxP entire sequence. Binding of neutralizing anti-TsNTxP rabbit antibodies to spotted peptides revealed GREGYPADGGGLPDSVKI as the more reactive peptide sequence. This epitope was made from the first eight residues of the protein (GREGYPAD) and from residues 47 to 54 (GLPDSVKI) of the C-terminal part of TsNTxP. BALB/c mice were immunized with synthetic GREGYPADGGGLPDSVKI peptide conju...Continue Reading

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Citations

Mar 20, 2012·Journal of Immunotoxicology·Manuela Berto PuccaJosé Elpidio Barbosa
Sep 29, 2011·Vaccine·Boussag-Abib Lila, Fatima Laraba-Djebari
Aug 6, 2014·Toxicon : Official Journal of the International Society on Toxinology·A O CarmoE Kalapothakis
Mar 31, 2015·Toxicon : Official Journal of the International Society on Toxinology·T M MendesC Chávez-Olórtegui
Sep 24, 2013·Toxicon : Official Journal of the International Society on Toxinology·M KhoobdelS Akbari
Feb 24, 2015·Toxicon : Official Journal of the International Society on Toxinology·A O CarmoE Kalapothakis
Feb 20, 2014·PLoS Neglected Tropical Diseases·Carolina Campolina Rebello HortaEvanguedes Kalapothakis
Jul 28, 2016·Toxins·Andreas Hougaard LaustsenMikael Engmark
Nov 8, 2018·Toxins·Erick Bermúdez-MéndezAndreas Hougaard Laustsen
May 29, 2020·International Journal of Biological Macromolecules·Patrícia D Vaz de MeloCarlos Chavez-Olortegui

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