In vivo quantification of endotoxin-induced nitric oxide production in pigs from Na15NO3-infusion

British Journal of Pharmacology
B SantakJ Vogt

Abstract

1. In this investigation the NO production rate is quantified in the pig during normotensive endotoxin-induced shock with increased cardiac output and during subsequent treatment with the NO synthase inhibitor N omega-monomethy-L-arginine (L-NMMA). NO production rate was derived from the plasma isotope-enrichment of 15N-labelled nitrate (15NO3-). 2. Three groups of animals (control, n = 5; endotoxin, n = 6; endotoxin + L-NMMA, n = 6) were anaesthetized and instrumented for the measurement of systemic and pulmonary haemodynamics. Each animal received a primed-continuous infusion of stable, non-radioactively labelled Na15 NO3 (bolus 30 mg, infusion rate 2.1 mg h-1). Arterial blood samples were taken 5, 10, 15, 30, 60 and 90 min later and every 90 minutes until the end of the experiment. 3. Continuous i.v. infusion of endotoxin was incrementally adjusted until mean pulmonary artery pressure (PAP) reached 50 mmHg and subsequently titrated to keep mean PAP approximately 35 mmHg. Hydroxyethylstarch was administered as required to maintain mean arterial pressure (MAP) > 60 mmHg. Six hours after the start of the endotoxin continuous i.v. L-NMMA (1 mg kg-1 h-1) was administered to the endotoxin + L-NMMA group. Haemodynamic data were mea...Continue Reading

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Citations

Dec 18, 2001·Pharmacology & Therapeutics·F FeihlL Liaudet
Dec 6, 2002·British Journal of Pharmacology·Maaike J BruinsNicolaas E P Deutz
Dec 1, 2004·Nutrition Research Reviews·Yvonne L J VissersNicolaas E P Deutz
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Jun 30, 2007·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Masahide Yoshida
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Aug 17, 2006·Shock·Yoram VodovotzGilles Clermont
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