In vivo recellularization of plain decellularized xenografts with specific cell characterization in the systemic circulation: histological and immunohistochemical study

Artificial Organs
Kazuyoshi TakagiShigeaki Aoyagi

Abstract

Decellularized biological scaffolds have been used for the tissue engineering of heart valves with good results in the pulmonary circulation. However, little information is available on the recellularization of plain decellularized biological scaffolds in the systemic circulation. The aim of this study was to determine whether plain decellularized xenografts (PDXs) can recellularize with specific cell characterization in the systemic circulation. The xenogenic aortic valved conduit grafts of rabbits were implanted in the abdominal aorta of dogs after decellularization. The grafts were explanted at 4, 12, or 24 weeks after implantation for histological, immunohistochemical examination, scanning electron microscope, and Western blot analysis. Although the valvular structures were completely lost after implantation, supravalvular conduits showed normal shapes and endothelialization. The PDXs were repopulated with basic vascular cell components in approximate natural proportions without immunological responses. The PDXs had been recellularized with specific cell characterization in the systemic circulation.

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Citations

Sep 12, 2008·Tissue Engineering. Part a·Seung-Woo ChoByung-Soo Kim
Mar 9, 2007·Artificial Organs·Paul S Malchesky
Jun 2, 2015·Expert Opinion on Biological Therapy·Daniel Y CheungJonathan T Butcher
May 8, 2009·Expert Review of Medical Devices·Anita MolFrank P T Baaijens
Sep 12, 2017·Journal of Tissue Engineering·Mitchell C VeDepoGabriel L Converse
Apr 26, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Helga BergmeisterGuenter Weigel
May 8, 2009·The Journal of Veterinary Medical Science·Yuuto SaidaYoshihisa Yamane
Sep 11, 2020·Nature Reviews. Cardiology·Emanuela S FiorettaMaximilian Y Emmert
Oct 15, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jie LiaoXiaoming Li
Apr 12, 2021·The Canadian Journal of Cardiology·Bahram MiraniCraig A Simmons

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