In vivo reconstitution of autophagy in Saccharomyces cerevisiae.

The Journal of Cell Biology
Yang CaoDaniel J Klionsky

Abstract

Autophagy is a major intracellular degradative pathway that is involved in various human diseases. The role of autophagy, however, is complex; although the process is generally considered to be cytoprotective, it can also contribute to cellular dysfunction and disease progression. Much progress has been made in our understanding of autophagy, aided in large part by the identification of the autophagy-related (ATG) genes. Nonetheless, our understanding of the molecular mechanism remains limited. In this study, we generated a Saccharomyces cerevisiae multiple-knockout strain with 24 ATG genes deleted, and we used it to carry out an in vivo reconstitution of the autophagy pathway. We determined minimum requirements for different aspects of autophagy and studied the initial protein assembly steps at the phagophore assembly site. In vivo reconstitution enables the study of autophagy within the context of the complex regulatory networks that control this process, an analysis that is not possible with an in vitro system.

References

Oct 6, 1998·Nature·N MizushimaY Ohsumi
Dec 2, 2000·Nature·Y IchimuraY Ohsumi
Apr 20, 2001·The Journal of Cell Biology·J KimD J Klionsky
Jun 30, 2001·Molecular Cell·S V ScottD J Klionsky
Dec 14, 2002·Developmental Cell·Takahiro ShintaniDaniel J Klionsky
Apr 8, 2004·Developmental Cell·Beth Levine, Daniel J Klionsky
Apr 23, 2004·The EMBO Journal·Stephen K DoveMark A Lemmon
May 13, 2004·The Journal of Biological Chemistry·Takahiro Shintani, Daniel J Klionsky
Jul 28, 2004·The Journal of Biological Chemistry·Yoshinobu IchimuraYoshinori Ohsumi
Aug 12, 2004·Nucleic Acids Research·Lei ZhengJean-Louis Reymond
Dec 24, 2004·Journal of Cell Science·Daniel J Klionsky
Jan 22, 2005·Molecular Biology of the Cell·Tomohiro Yorimitsu, Daniel J Klionsky
May 20, 2005·Molecular Biology of the Cell·Heesun CheongDaniel J Klionsky
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens
Dec 8, 2005·The Journal of Biological Chemistry·Jun-ichi IwataEiki Kominami
Sep 12, 2006·Autophagy·Yufang ShaoXuejun Jiang
Feb 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kuninori SuzukiYoshinori Ohsumi
Mar 17, 2007·Biochemical and Biophysical Research Communications·Yukiko KabeyaYoshinori Ohsumi
Mar 31, 2007·Nature Reviews. Drug Discovery·David C RubinszteinDaniel J Klionsky
Aug 23, 2007·Nature Reviews. Molecular Cell Biology·Daniel J Klionsky
Sep 26, 2007·Cell Research·Yang Cao, Daniel J Klionsky
Nov 8, 2007·The Journal of Biological Chemistry·Takao HanadaYoshinori Ohsumi
Nov 17, 2007·Genes & Development·Noboru Mizushima
Nov 28, 2007·The Journal of Biological Chemistry·Yuko FujiokaFuyuhiko Inagaki
Feb 22, 2008·Molecular Biology of the Cell·Tomoko KawamataYoshinori Ohsumi

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Citations

Jun 6, 2009·Nature Reviews. Molecular Cell Biology·Hitoshi NakatogawaYoshinori Ohsumi
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael J Kershaw, Nicholas J Talbot
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kai MaoDaniel J Klionsky
Feb 16, 2010·The Journal of Biological Chemistry·Usha NairDaniel J Klionsky
Jul 17, 2009·Molecular Biology of the Cell·Taras Y NazarkoSuresh Subramani
Dec 14, 2011·Biochemistry. Biokhimii︠a︡·A A Sibirny
Apr 5, 2014·Nature Structural & Molecular Biology·Daniel J Klionsky, Brenda A Schulman
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Apr 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Molly A Lynch-DayDaniel J Klionsky
Nov 28, 2009·Nutrition Reviews·Martha H Stipanuk
Jun 15, 2014·FEBS Letters·Meiyan Jin, Daniel J Klionsky
Jan 12, 2011·The Journal of Cell Biology·Eisuke Itakura, Noboru Mizushima
Jan 13, 2010·The Journal of Cell Biology·Wei-Lien YenDaniel J Klionsky
Feb 26, 2016·Journal of Molecular Biology·Xin Wen, Daniel J Klionsky
Nov 14, 2015·Autophagy·Elizabeth Delorme-Axford, Daniel J Klionsky
Jun 12, 2009·Current Opinion in Cell Biology·Jean-Claude FarréMichael Thumm
Nov 12, 2014·Traffic·Steven K BackuesDaniel J Klionsky
Nov 7, 2009·The EMBO Journal·Jemma L Webber, Sharon A Tooze
Jun 17, 2010·IUBMB Life·Sharon A ToozeJemma L Webber
Mar 3, 2009·BMB Reports·Meenakshi Dwivedi, Joohong Ahnn
Oct 26, 2011·Autophagy·Andy BassDaniel J Klionsky
Jun 22, 2012·Autophagy·Steven K Backues, Daniel J Klionsky
Jan 14, 2012·Autophagy·Hitoshi NakatogawaYoshinori Ohsumi
Apr 7, 2009·Biochimica Et Biophysica Acta·Eduardo Cebollero, Fulvio Reggiori
Jun 5, 2013·Genetics·Fulvio Reggiori, Daniel J Klionsky
Mar 23, 2021·The FEBS Journal·Daniel Hamaoui, Agathe Subtil

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Methods Mentioned

BETA
fluorescence microscopy
lipidation
gene knockouts
PCR
immunoprecipitation

Software Mentioned

DeltaVision softWoRx

Related Concepts

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