PMID: 11327335May 1, 2001Paper

In vivo recording of monophasic action potentials in awake dogs--new applications for experimental electrophysiology

Basic Research in Cardiology
L EckardtW Haverkamp

Abstract

Despite enormous developments in the field of clinical and experimental electrophysiology there is still a gap in evaluating repolarization in the awake animal. Numerous previous studies have used monophasic action potentials (MAP) to assess repolarization in vitro and in vivo in anesthetized animal models. However, an approach for recording MAP in awake dogs without interference of anesthesia has not yet been developed. We developed an experimental technique to record MAP in conscious dogs by means of conventional rubber introducers which were implanted into the internal jugular vein. In seven awake dogs, atrial as well as ventricular MAP were simultaneously measured without complications. Pacing thresholds were low and stable over time ranging from 0.2 to 4.0 mA. The MAP amplitudes ranged from 10 to 30 mV for ventricular and from 5 to 15 mV for atrial MAP. Continuous MAP recordings of stable amplitude could be made from the same endocardial site for periods of up to one hour. Antegrade and retrograde AV-nodal conduction properties could be assessed. Programmed stimulation was performed to simultaneously determine local refractory periods and MAP duration at cycle lengths from 500 to 200 ms. In awake, unsedated dogs the measur...Continue Reading

Citations

Jun 7, 2013·Herzschrittmachertherapie & Elektrophysiologie·Sven KaeseLars Eckardt
May 3, 2003·Methods : a Companion to Methods in Enzymology·Lars EckardtWilhelm Haverkamp

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