In vivo red blood cell sickling and mechanism of recovery in whiting, Merlangius merlangus

The Journal of Experimental Biology
Pia Koldkjaer, Michael Berenbrink


Haemoglobin concentrations in vertebrate red blood cells are so high that in human sickle cell disease a single surface amino acid mutation can result in formation of large insoluble haemoglobin aggregates at low oxygen levels, causing peculiar cell deformations or 'sickling'. This may cause vascular occlusion and thereby severe pain, organ failure and death. Here, using light and transmission electron microscopy, we demonstrate extensive in vivo sickling of whiting red blood cells after capture stress without any apparent haemolysis and show its subsequent recovery. We show exceptionally high cooperative proton binding during the sickling process in vitro and identify the reduction of extracellular pH below resting values as the primary cause for in vivo sickling, although the response is modulated to a lesser extent also by oxygen tension. Using isotope tracer fluxes, we further show that beta-adrenergic hormones, which are released under capture stress, activate a powerful endogenous Na/H exchanger in these fish red blood cells, which is known to elevate intracellular pH. beta-adrenergic treatment further leads to a marked reduction of acid-induced in vitro sickling, which is impaired when Na/H exchange is inhibited by amilo...Continue Reading


Mar 1, 1990·Respiration Physiology·H KobayashiP Scheid
Jul 1, 1985·Journal of Ultrastructure Research·X MatteiC Mattei
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J HofrichterW A Eaton
Apr 1, 1968·The Journal of Experimental Medicine·J Döbler, J F Bertles
Sep 11, 1997·The New England Journal of Medicine·H F Bunn
Sep 18, 1998·The Biological Bulletin·F I HárosiJ R Van Keuren
Apr 7, 2000·Advances in Parasitology·A J Davies, M R Johnston
Aug 12, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S EggintonC Skilbeck
Feb 13, 2001·British Journal of Haematology·G R Serjeant
Mar 20, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·P K Knudsen, F B Jensen
Jul 4, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Stuart Egginton
Jul 23, 1960·Nature·E UNDRIZH LAHMANN
Oct 21, 1950·Nature·M F PERUTZ, J M MITCHISON
Nov 16, 2004·Biophysical Journal·Garrott W ChristophWilliam A Eaton
Feb 17, 2006·Respiratory Physiology & Neurobiology·Michael Berenbrink
May 24, 2006·The Journal of Biological Chemistry·Cinzia VerdeGuido di Prisco

❮ Previous
Next ❯


Feb 22, 2011·Proteins·Mitra S Rana, Austen F Riggs
Jul 20, 2018·Experimental Physiology·Benjamin ChatelDavid Bendahan
Sep 13, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Pia KoldkjaerMichael Berenbrink
May 31, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roy E WeberThomas A Gorr

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.


Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.