In vivo regulation of cytostatic activity in Xenopus metaphase II-arrested oocytes

Developmental Biology
C ThibierR Ozon

Abstract

Metaphase II arrest of Xenopus oocyte is characterized by the presence of M-phase-promoting factor (MPF) and of a microtubular spindle, both of which are stable in the presence of protein synthesis inhibitors. We studied in vivo this equilibrium state that is settled during meiotic maturation. At time of germinal vesicle breakdown (GVBD), cdc2 kinase and MAP kinase activities are stimulated. A component of the cyclin ubiquitin ligase, CDC27, is phosphorylated at the same time and remains phosphorylated until fertilization, indicating that an important component of the ligase complex is modified as early as GVBD. During a first period extending from GVBD until the cortical anchorage of the metaphase II spindle, homogeneous pools of cdc2 kinase and mitogen-activated protein (MAP) kinase activities are present in oocyte and are strictly dependent on protein turnover, since protein synthesis inhibition induces their total inactivation and drives oocytes into interphase. The metaphase II spindle, once anchored into the cortex, is no more sensitive to protein synthesis inhibition, likewise MAP kinase activity. During this cellular arrest, cdc2 kinase is divided into two distinctly regulated pools. The first one contains cyclin B that...Continue Reading

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Citations

Oct 10, 1997·Molecular Reproduction and Development·F TaiebC Jessus
Mar 10, 2004·The International Journal of Biochemistry & Cell Biology·Naoyuki SakaiHitoshi Sawada
Aug 31, 1999·Development, Growth & Differentiation·T IwamatsuM Yamashita
Oct 26, 2002·Cloning and Stem Cells·Kazuhiro KikuchiHideaki Tojo
Mar 1, 2005·Developmental Biology·Tomomi M YamamotoTakeo Kishimoto
Dec 5, 2006·Developmental Biology·Munemichi OheNoriyuki Sagata
Nov 12, 2002·The Journal of Biological Chemistry·Anna CastroSimon Galas
Mar 26, 2003·Genes & Development·Brian J Tunquist, James L Maller
Oct 1, 2011·The International Journal of Biochemistry & Cell Biology·Gaëlle MarteilJacek Z Kubiak

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