In vivo regulation of glutamine synthetase activity in the marine chlorophyll b-containing cyanobacterium Prochlorococcus sp. strain PCC 9511 (oxyphotobacteria)

Applied and Environmental Microbiology
S El AlaouiJose Manuel García-Fernández

Abstract

The physiological regulation of glutamine synthetase (GS; EC 6.3.1.2) in the axenic Prochlorococcus sp. strain PCC 9511 was studied. GS activity and antigen concentration were measured using the transferase and biosynthetic assays and the electroimmunoassay, respectively. GS activity decreased when cells were subjected to nitrogen starvation or cultured with oxidized nitrogen sources, which proved to be nonusable for Prochlorococcus growth. The GS activity in cultures subjected to long-term phosphorus starvation was lower than that in equivalent nitrogen-starved cultures. Azaserine, an inhibitor of glutamate synthase, provoked an increase in enzymatic activity, suggesting that glutamine is not involved in GS regulation. Darkness did not affect GS activity significantly, while the addition of diuron provoked GS inactivation. GS protein determination showed that azaserine induces an increase in the concentration of the enzyme. The unusual responses to darkness and nitrogen starvation could reflect adaptation mechanisms of Prochlorococcus for coping with a light- and nutrient-limited environment.

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Citations

Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Jose Manuel García-FernándezJesús Diez
Oct 23, 2008·PloS One·Guadalupe Gómez-BaenaJose Manuel García-Fernández
Jul 26, 2014·PloS One·María Agustina Domínguez-MartínJosé Manuel García-Fernández
Feb 1, 2009·Environmental Microbiology Reports·Oriol Alberto RangelJose Manuel García-Fernández
Apr 1, 2012·Environmental Microbiology Reports·Brian McDonaghJose M García Fernández
Nov 30, 2004·Research in Microbiology·Jose M García-Fernández, Jesús Diez
May 29, 2012·Biotechnology Advances·Song QinPeng Jiang
Aug 1, 2003·FEMS Microbiology Ecology·Sushanta Kumar SahaGopalakrishnan Subramanian
Aug 1, 2002·FEMS Microbiology Ecology·Antonio López-LozanoJose Manuel García-Fernández
Jul 23, 2016·Frontiers in Microbiology·María Agustina Domínguez-MartínJosé M García-Fernández
Jun 9, 2017·MSystems·Maria Agustina Domínguez-MartínJosé Manuel García-Fernández
Oct 4, 2006·Molecular Systems Biology·Andrew C TolonenSallie W Chisholm
Apr 1, 2002·FEMS Microbiology Ecology·David J Scanlan, Nyree J West
Dec 22, 2017·FEMS Microbiology Letters·María Agustina Domínguez-MartínJosé Manuel García-Fernández
Jan 30, 2018·Frontiers in Microbiology·María A Domínguez-MartínJosé M García-Fernández
Mar 25, 2017·Frontiers in Microbiology·María Del Carmen Muñoz-MarínJosé M García-Fernández
Mar 6, 2019·MSystems·Martin J SzulErik R Zinser
Jan 17, 2019·PeerJ·José Ángel Moreno-CabezueloJosé Manuel García-Fernández
Oct 31, 2018·Life·Paul BolayStephan Klähn

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