PMID: 1212655Dec 1, 1975Paper

In vivo repair of rat intestinal DNA damage by alkylating agents

Cancer
K Kanagalingam, M E Balis

Abstract

In an effort to evaluate the possible correlation of the transforming ability of the known colon carcinogens dimethylhydrazine, 3-2'-dimethyl-4-aminobiphenyl, and methylazoxymethanolacetate to damage and repair of DNA, a series of compounds known to react with DNA-nitrogen mustard, methylmethanesulfonate, and mitomycin C--were administered to rats that had been prelabeled with 3H-thymidine. The DNA of crypt and villus of the jejunum and crypt and surface cells of the large bowel were analyzed by ultracentrifugation on an alkaline sucrose gradient. All fractions suffered degradation to such an extent that essentially no undamaged DNA was detectable. This was followed by repair and an increase in size. However, in the surface cells of the colon of animals that had received a carcinogenic insult there was far less rapid repair. Since this is the site where tumors would ultimately arise these data are supportive of the hypothesis that there is a relationship between decreased repair and carcinogenicity. In view of the age related incidence of colon cancer, repair in older animals was evaluated and was found to be less than that seen in the young. Since multiple treatment with the carcinogen dimethylhydrazine is required and there i...Continue Reading

References

Dec 1, 1969·Experimental Cell Research·A R ImondiM Lipkin
Nov 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·R B SetlowW L Carrier

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Citations

Jan 1, 1984·Environmental Mutagenesis·G R Hoffmann, R W Morgan
Apr 29, 1998·Diseases of the Colon and Rectum·A Banerjee, P Quirke

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