In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts

Scientific Reports
Hyun Woo ChoiJeong Tae Do

Abstract

Recently, induced pluripotent stem cells (iPSCs) have been generated in vivo from reprogrammable mice. These in vivo iPSCs display features of totipotency, i.e., they differentiate into the trophoblast lineage, as well as all 3 germ layers. Here, we developed a new reprogrammable mouse model carrying an Oct4-GFP reporter gene to facilitate the detection of reprogrammed pluripotent stem cells. Without doxycycline administration, some of the reprogrammable mice developed aggressively growing teratomas that contained Oct4-GFP(+) cells. These teratoma-derived in vivo PSCs were morphologically indistinguishable from ESCs, expressed pluripotency markers, and could differentiate into tissues of all 3 germ layers. However, these in vivo reprogrammed PSCs were more similar to in vitro iPSCs than ESCs and did not contribute to the trophectoderm of the blastocysts after aggregation with 8-cell embryos. Therefore, the ability to differentiate into the trophoblast lineage might not be a unique characteristic of in vivo iPSCs.

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Citations

Jan 6, 2016·Stem Cells and Development·Jong Soo KimJeong Tae Do
Jul 16, 2014·Biochemical and Biophysical Research Communications·Kotaro OhnishiYasuhiro Yamada
Jun 12, 2016·Trends in Molecular Medicine·Clara Soria-Valles, Carlos López-Otín
May 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sangeetha Vadakke-MadathilHina W Chaudhry

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
FACS
genotyping
PCR
electrophoresis

Software Mentioned

Illumina GenomeStudio
FACSDiva

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