In vivo residue-specific histone methylation dynamics.

The Journal of Biological Chemistry
Barry M ZeeBenjamin A Garcia

Abstract

Methylation of specific histone residues is capable of both gene activation and silencing. Despite vast work on the function of methylation, most studies either present a static snapshot of methylation or fail to assign kinetic information to specific residues. Using liquid chromatography-tandem mass spectrometry on a high-resolution mass spectrometer and heavy methyl-SILAC labeling, we studied site-specific histone lysine and arginine methylation dynamics. The detection of labeled intermediates within a methylation state revealed that mono-, di-, and trimethylated residues generally have progressively slower rates of formation. Furthermore, methylations associated with active genes have faster rates than methylations associated with silent genes. Finally, the presence of both an active and silencing mark on the same peptide results in a slower rate of methylation than the presence of either mark alone. Here we show that quantitative proteomic approaches such as this can determine the dynamics of multiple methylated residues, an understudied portion of histone biology.

References

Nov 1, 1978·Cell·A WorcelM L Wong
Jan 1, 1991·Annual Review of Biochemistry·M A Osley
Feb 1, 1972·Archives of Biochemistry and Biophysics·P ByvoetB J Noland
Aug 16, 1983·Biochemistry·L P DjondjurovE C Ivanova
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·A M SirotkinA I Skoultchi
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y OdaB T Chait
Dec 29, 2000·Nature·T MisteliD T Brown
Aug 11, 2001·Science·T Jenuwein, C D Allis
Oct 10, 2002·Cell·Raymond C TrievelJames H Hurley
Mar 22, 2003·Science·Kathrin PlathYi Zhang
Oct 3, 2003·Nature·Wolfgang FischleC David Allis
Dec 24, 2003·Molecular Cell·Antoine H F M PetersThomas Jenuwein
May 18, 2004·Genes & Development·Gunnar SchottaThomas Jenuwein
Jun 10, 2004·Cell·Siavash K KurdistaniMichael Grunstein
Oct 16, 2004·The Journal of Biological Chemistry·Debasis PatnaikSriharsa Pradhan
Mar 23, 2005·Nature Methods·Shao-En OngMatthias Mann
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Cyrus Martin, Yi Zhang
Jun 2, 2006·Genes & Development·Barna D FodorThomas Jenuwein
Sep 22, 2006·The Journal of Biological Chemistry·Federico FornerisAndrea Mattevi
Jan 16, 2007·Molecular Cell·Yang Shi, Johnathan R Whetstine
Feb 27, 2007·Cell·Tony Kouzarides
Mar 10, 2007·Science·Yoshiko MitoSteven Henikoff
Apr 21, 2007·Nature Protocols·Benjamin A GarciaDonald F Hunt
May 29, 2007·Nature Methods·Benjamin A GarciaNeil L Kelleher
Jun 2, 2007·Cell·Patrick TrojerDanny Reinberg
Oct 31, 2007·Molecular and Cellular Biology·James J PesaventoCraig A Mizzen
Feb 21, 2008·Molecular and Cellular Biology·David J StegerChristopher R Vakoc
Dec 5, 2008·Journal of Proteome Research·Michael T BoyneNeil L Kelleher
Mar 10, 2009·Nature Structural & Molecular Biology·Antonis KirmizisTony Kouzarides
Apr 22, 2009·Science in China. Series C, Life Sciences·Fei Lan, Yang Shi
Jul 2, 2009·Molecular & Cellular Proteomics : MCP·Gary LeRoyBenjamin A Garcia
Jul 23, 2009·Journal of Cellular Biochemistry·Benjamin M Lee, Louis C Mahadevan

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Citations

Feb 16, 2011·Cell Research·Bing Zhu, Danny Reinberg
Jun 7, 2013·Journal of Human Genetics·Hiroshi Kimura
Jan 21, 2011·Nature·Raphaël Margueron, Danny Reinberg
Jul 23, 2013·Nature Immunology·Silvia Monticelli, Gioacchino Natoli
Jul 5, 2013·Nature Reviews. Cancer·Jason W Locasale
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yupeng ZhengNeil L Kelleher
Aug 12, 2010·The Journal of Biological Chemistry·Steve M M SweetNeil L Kelleher
Jan 18, 2011·The Journal of Biological Chemistry·Wen YuanBing Zhu
Feb 13, 2013·Briefings in Functional Genomics·Till BartkePeter A DiMaggio
Mar 23, 2013·Nucleic Acids Research·Anne Katrine Alsing, Kim Sneppen
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Chang HuangBing Zhu
May 2, 2012·Molecular and Cellular Biology·Barry M ZeeBenjamin A Garcia
Dec 8, 2010·Epigenetics & Chromatin·Barry M ZeeBenjamin A Garcia
Oct 21, 2010·Genome Biology·Roger B Deal, Steven Henikoff
Nov 14, 2013·PloS One·Wai Lim KuEdward Ott
Mar 16, 2012·Future Medicinal Chemistry·F CherblancM J Fuchter
Nov 20, 2014·Epigenetics & Chromatin·Lisette C M Anink-GroenenFrank J Bruggeman
Jul 12, 2011·Nature Chemical Biology·Masoud VedadiJian Jin
Mar 14, 2013·The Journal of Biological Chemistry·Adam G EverttsBenjamin A Garcia
Oct 18, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Yue-Qiang ZhaoVictoria V Lunyak
Aug 24, 2010·Amino Acids·Ry Y Tweedie-Cullen, Isabelle M Mansuy
Apr 25, 2012·Analytical and Bioanalytical Chemistry·Shao-En Ong
Feb 14, 2013·FEBS Letters·Adam P LothropStephen M Fuchs
Sep 25, 2015·Expert Review of Proteomics·Özlem ÖnderBenjamin A Garcia
Nov 27, 2010·FEBS Letters·Annette N D Scharf, Axel Imhof
Jun 20, 2012·FEBS Letters·Joshua A JadwinKazuya Machida
Jan 6, 2016·Chromosoma·Hanneke Vlaming, Fred van Leeuwen
Jan 25, 2014·Biochimica Et Biophysica Acta·Gang Yuan, Bing Zhu
Apr 9, 2011·Trends in Cell Biology·Izumi V Hinkson, Joshua E Elias
Aug 6, 2010·Trends in Biochemical Sciences·Teresa K Barth, Axel Imhof
Nov 13, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S R GadhiaF A Barile
Dec 8, 2015·Cell Stem Cell·James G RyallVittorio Sartorelli
May 11, 2015·Progress in Biophysics and Molecular Biology·Kimberly R Shorter, Brooke H Miller
Jun 15, 2012·Proteomics·Andrea M BrunnerIsabelle M Mansuy
Jul 16, 2011·EMBO Reports·Dirk De VosFred van Leeuwen
Jun 12, 2012·European Journal of Immunology·Sanmay BandyopadhyayFernando Macian

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