In vivo selection of a hepatitis B virus mutant with abnormal viral protein expression

The Journal of General Virology
D KremsdorfC Brechot

Abstract

We have investigated the molecular basis for the in vivo selective advantage of a hepatitis B virus (HBV) mutant. We have determined the complete nucleotide sequences of the major HBV forms identified at the beginning (B1-83) and end (B1-89) of a 6 year follow-up of a chronically infected patient. The B1-89 sequence showed marked nucleotide rearrangements (a nucleotide divergence of 11.3 % compared with the adw2 subtype), but sequence comparison showed that both viral molecules were of common origin (62/138 mutations were found on both molecules, compared to adw2). In vitro transfection of Huh7 cells showed important modifications in B1-89 viral protein expression. We observed a decrease in B1-89 envelope protein expression associated with a modification of the migration pattern of the large envelope protein. For the B1-89 capsid protein, an insertion of 36 nucleotides at the 5' end of the C gene resulted in increased expression of a core-specific protein of abnormal size (24 kDa versus 22 kDa). Finally, our data also suggest an increase in the transcomplementation efficiency of the mutated B1-89 polymerase protein. Thus, we were able to demonstrate distinct intrinsic properties of HBV DNA molecules isolated from a chronic carr...Continue Reading

Citations

Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N OgataR H Purcell
Mar 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hideaki KatoMasashi Mizokami
Mar 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masaya SugiyamaMasashi Mizokami
Mar 9, 2005·Vaccine·Anna KramvisGuido François
Jul 13, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stephan Günther
Nov 8, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Magnus Lindh
Jan 18, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Bernard Weber
Jun 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael ChudyC Micha Nübling
Feb 22, 2005·Journal of Viral Hepatitis·S Schaefer
Dec 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Isabelle CheminChristian Trépo
Apr 1, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Michael BasicKai-Henrik Peiffer

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