In vivo selection of aac(6')-Ib-cr and mutations in the gyrA gene in a clinical qnrS1-positive Salmonella enterica serovar Typhimurium DT104B strain recovered after fluoroquinolone treatment

The Journal of Antimicrobial Chemotherapy
María de ToroYolanda Sáenz

Abstract

To characterize the mechanisms implicated in the in vivo selection of quinolone and aminoglycoside resistance in a faecal Salmonella enterica serovar Typhimurium DT104B strain recovered after ciprofloxacin treatment of a hospitalized elderly patient with acute gastroenteritis. Two Salmonella Typhimurium isolates were obtained before (Se6) and after (Se20) treatment and they were typed by PFGE and multilocus sequence typing (MLST). Antimicrobial susceptibility was determined by disc diffusion and agar dilution methods. Class 1, 2 and 3 integrons and resistance mechanisms were studied by PCR and sequencing. Plasmids were typed. Both Salmonella Typhimurium strains were resistant to tetracycline, streptomycin and sulphonamides, while Se20 was also resistant to nalidixic acid, ciprofloxacin, norfloxacin, levofloxacin, ofloxacin, amikacin, tobramycin, kanamycin and trimethoprim. PFGE and MLST showed a clonal relationship between the strains, which belonged to the sequence type ST36. Both strains contained the repC-sul2-strA-strB structure and tet(A) and qnrS1 genes, and strain Se20 also contained the aac(6')-Ib-cr gene, the Ser83-->Tyr substitution in GyrA and one class 1 integron with the dfrA17 + aadA5 gene cassette arrangement lac...Continue Reading

References

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Citations

Jul 4, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Romney M HumphriesJanet A Hindler
Jan 31, 2013·The Journal of Antimicrobial Chemotherapy·María de ToroYolanda Sáenz
Nov 23, 2011·Antimicrobial Agents and Chemotherapy·Beatriz Rojo-BezaresYolanda Sáenz
Sep 10, 2014·International Journal of Molecular Sciences·Susana CorreiaGilberto Igrejas
Apr 21, 2012·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Dan I Andersson, Diarmaid Hughes
Dec 3, 2014·Plasmid·M Pilar Garcillán-BarciaLuis Martínez-Martínez
Jul 27, 2012·International Journal of Antimicrobial Agents·Joaquim RuizCláudia Gomes
Oct 12, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ezequiel AlbornozAlejandro Petroni
Jan 21, 2017·Genes·Meredith SchroederAmanda E Brooks
May 28, 2014·Revista Da Sociedade Brasileira De Medicina Tropical·Alexsandra Maria Silva LimaAna Catarina Souza Lopes
Apr 17, 2019·Journal of Medical Microbiology·Megha KaushikPooja Gulati
Aug 16, 2019·Clinical Microbiology Reviews·Joaquim Ruiz
Jun 25, 2015·Microbiology Spectrum·María de ToroFernando De La Cruz
Jan 15, 2015·Microbiology Spectrum·George A JacobyDavid C Hooper

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