PMID: 6166616May 1, 1981Paper

In vivo staining of cytoskeletal actin by autointernalization of nontoxic concentrations of nitrobenzoxadiazole-phallacidin

The Journal of Cell Biology
L S BarakW W Webb

Abstract

The blue light-excited fluorescent phallotoxin derivative nitrobenzoxadiazole phallacidin (NBD-Ph) was used to stain entire tissue culture monolayers of live L6 mouse cells and other mammalian cell lines without the aid of permeabilization treatment. Although cells tend to exclude the fluorescent toxin, reducing the internal concentration by approximately 1,000 times, some of it enters the cells, probably by pinocytosis, and stains actin structures at low intracellular NBD-Ph concentrations (approximately 5-15 nM), where cell toxicity was negligible or at least not detectable by phase-contrast microscopy. Protracted treatments with NBD-Ph did induce pharmacological responses similar to those of phalloidin. The dissociation constant for NBD-Ph with F-actin in fixed and extracted L6 cells was determined, from staining intensity measurements at various NBD-Ph concentrations, to be 1.5-2.5 x 10(-8) M.

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Citations

Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G A Upadhya, S M Strasberg
Jul 1, 1992·Molecular Reproduction and Development·N H HartJ S Wolenski
Jul 13, 2001·Cell Biology International·R G WilsonA M Zimmerman
Jun 1, 1988·Developmental Biology·J H Henson, D A Begg
Jan 1, 1990·Neurochemistry International·S Abe-DohmaeR Tanaka
Jan 1, 1983·Experimental Gerontology·M RaesJ Remacle
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·S R Gordon
Feb 1, 2012·Bioconjugate Chemistry·Jessie A KeyChristopher W Cairo
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E S WuW W Webb
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I MabuchiT Sawai
Sep 1, 1981·The Journal of Cell Biology·W W CarleyW W Webb
Dec 1, 1982·The Journal of Cell Biology·L S Barak, W W Webb
Jan 1, 1984·The Journal of Cell Biology·P SondereggerP G Nelson
Jun 27, 2002·Transplantation·G Aravinda Upadhya, Steven M Strasberg
Jun 1, 1991·The Journal of Clinical Investigation·M ShapiroJ L Madara
Apr 18, 2000·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Opas
Jan 1, 1986·Cell Motility and the Cytoskeleton·H Schatten, G Schatten
Jan 1, 1983·Cell Motility·C A ClineG Schatten
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Jun 13, 2015·Chembiochem : a European Journal of Chemical Biology·Lucia CardoStephen P Watson
Dec 31, 2011·The Journal of Biological Chemistry·Hyun-Jeong YangMitsutoshi Setou
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Sep 1, 1989·The American Journal of Physiology·P G PhillipsM F Tsan
Jan 1, 1991·The American Journal of Physiology·G H DingR M Hays

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