In vivo study on the neurotransmitters and their metabolites change in depressive disorder rat plasma by ultra high performance liquid chromatography coupled to tandem mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Longshan ZhaoChunfu Wu

Abstract

A sensitive and versatile, ultra-high performance, liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method coupled to pre-column derivatization for the simultaneous determination of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), dopamine (DA), norepinephrine (NE), homovanillic acid (HVA), γ-aminobutyric acid (GABA) and glutamic acid (Glu) was developed and validated in rat plasma. The analytes were dansylated under strong alkaline conditions after protein precipitation extraction, which were analyzed on a BEH C18 column using a gradient elution. The lower limit of quantification (LLOQ) values for 5-HT, 5-HIAA, DA, NE, HVA, GABA and Glu were 1.00, 1.00, 0.991, 0.992, 1.02, 1000, and 5030 pmol/mL, respectively. Good linearity was obtained (r > 0.99) and the intra- and inter-day precisions of the method (relative standard deviation, RSD%) were lower than 12%. The method was novel, sensitive and specific which can provide an alternative method for the quantification of neurotransmitters and their metabolites in plasma samples.

References

Jul 9, 2004·Archives of General Psychiatry·Gerard SanacoraGraeme F Mason
May 19, 2006·Progress in Neuro-psychopharmacology & Biological Psychiatry·Hideaki MitaniRyuzou Kawahara
Apr 18, 2009·European Journal of Clinical Pharmacology·Esra KüçükibrahimoğluM Zafer Gören
Jul 28, 2009·Progress in Neuro-psychopharmacology & Biological Psychiatry·Haixia DangYuqing Wang
Oct 6, 2009·Analytica Chimica Acta·Maura PerryRobert T Kennedy
Nov 17, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shuning ZhengFamei Li
Aug 11, 2010·Neuroscience and Biobehavioral Reviews·David T MarcKelly L Olson
Apr 10, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Guodong ZhangZhaosheng Lin
Jul 11, 2012·Neuroscience and Biobehavioral Reviews·Matthew N HillJoanne Weinberg
Apr 23, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Michel Hamon, Pierre Blier
Apr 17, 2014·Journal of Neuroscience Methods·Fei HuangXiao-jun Wu
Jun 1, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Ti-Fei Yuan, Burton M Slotnick
Jun 20, 2014·Experimental and Therapeutic Medicine·Xiao-Wei JiDian-Yun Wang
Dec 6, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Pei JiangMi-Mi Tang
Dec 30, 2014·Neuroscience and Biobehavioral Reviews·Amelia A AssarehPerminder S Sachdev
Dec 30, 2014·Neuroscience Letters·Mei-Ling JiangGuo-Qing Gong
Jan 18, 2015·Journal of Pharmaceutical and Biomedical Analysis·Nurullah ŞanlıCraig Lunte

❮ Previous
Next ❯

Citations

Jun 24, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marianne Skov-Skov BerghÅse Marit Leere Øiestad
Feb 10, 2016·Biomedical Chromatography : BMC·Yingfeng WangBaosheng Zhao
Aug 9, 2016·Auris, Nasus, Larynx·Sang Gyun JinShi Nae Park
Aug 11, 2016·Behavioural Brain Research·Naofumi KunisawaYukihiro Ohno
Mar 25, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hyun Ju ShinJongki Hong
Nov 2, 2016·Hormone Molecular Biology and Clinical Investigation·Nirja K Chaudhari, Laxmipriya P Nampoothiri
Dec 9, 2020·International Journal of Molecular Sciences·Olga V AverinaVladimir P Chekhonin
May 30, 2020·Journal of Pharmaceutical and Biomedical Analysis·Ana Sofia Abrantes DiasBruno Manadas
Oct 24, 2017·Analytical Chemistry·Víctor Pérez-FernándezMorven A Cameron

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.